Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   U0032_RS02050 Genome accession   NZ_CP140008
Coordinates   449408..450085 (-) Length   225 a.a.
NCBI ID   WP_000178163.1    Uniprot ID   -
Organism   Escherichia coli strain ATCC 11303     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444408..455085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0032_RS02035 (U0032_02035) chiA 445775..448468 (+) 2694 WP_000773143.1 bifunctional chitinase/lysozyme -
  U0032_RS02040 (U0032_02040) bfd 448637..448831 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  U0032_RS02045 (U0032_02045) bfr 448903..449379 (+) 477 WP_000675504.1 bacterioferritin -
  U0032_RS02050 (U0032_02050) pilD 449408..450085 (-) 678 WP_000178163.1 prepilin peptidase GspO Machinery gene
  U0032_RS02055 (U0032_02055) gspM 450085..450546 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  U0032_RS02060 (U0032_02060) gspL 450543..451706 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  U0032_RS02065 (U0032_02065) gspK 451721..452704 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  U0032_RS02070 (U0032_02070) gspJ 452697..453284 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  U0032_RS02075 (U0032_02075) gspI 453277..453654 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  U0032_RS02080 (U0032_02080) gspH 453651..454160 (-) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  U0032_RS02085 (U0032_02085) gspG 454168..454605 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24928.81 Da        Isoelectric Point: 7.9563

>NTDB_id=832956 U0032_RS02050 WP_000178163.1 449408..450085(-) (pilD) [Escherichia coli strain ATCC 11303]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTSAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=832956 U0032_RS02050 WP_000178163.1 449408..450085(-) (pilD) [Escherichia coli strain ATCC 11303]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTAGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378