Detailed information NTG0832950

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: U0032_RS00980
• Genome accession: NZ_CP140008
• Coordinates: 209227..210210 (+)
• Length: 327 a.a.
• NCBI ID: WP_001196486.1
• Uniprot ID: A7ZT85
• Organism: Escherichia coli strain ATCC 11303
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 204227..215210

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
U0032_RS00960 (U0032_00960)	dppA	205370..206977 (+)	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
U0032_RS00970 (U0032_00970)	dppB	207285..208304 (+)	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
U0032_RS00975 (U0032_00975)	dppC	208314..209216 (+)	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
U0032_RS00980 (U0032_00980)	amiE	209227..210210 (+)	984	WP_001196486.1	dipeptide ABC transporter ATP-binding protein	Regulator
U0032_RS00985 (U0032_00985)	dppF	210207..211211 (+)	1005	WP_000107012.1	dipeptide ABC transporter ATP-binding subunit DppF	-
U0032_RS00990 (U0032_00990)	yhjV	211241..212512 (-)	1272	WP_001295225.1	aromatic amino acid transport family protein	-
U0032_RS00995 (U0032_00995)	-	212988..213095 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
U0032_RS01000 (U0032_01000)	-	213471..213578 (+)	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
U0032_RS01005 (U0032_01005)	ldrD	213954..214061 (+)	108	WP_000141634.1	type I toxin-antitoxin system toxic polypeptide LdrD	-

Sequence

Protein

• Length: 327 a.a.
• Molecular weight: 35844.36 Da
• Isoelectric Point: 6.5814

>NTDB_id=832950 U0032_RS00980 WP_001196486.1 209227..210210(+) (amiE) [Escherichia coli strain ATCC 11303]
MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGSGKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISE
KERRNLVGAEVAMIFQDPMTSLNPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVAEAAHKIIVMYAGQVVETGDAHA
IFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPGKYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLD
DAGRPTL

Nucleotide

• Length: 984 bp

>NTDB_id=832950 U0032_RS00980 WP_001196486.1 209227..210210(+) (amiE) [Escherichia coli strain ATCC 11303]
ATGGCGTTATTAAATGTAGATAAATTATCGGTGCATTTCGGCGACGAAAGCGCGCCGTTCCGCGCCGTAGACCGCATCAG
CTACAGCGTAAAACAGGGCGAAGTGGTCGGGATTGTGGGTGAGTCCGGCTCCGGTAAGTCGGTCAGTTCACTGGCGATTA
TGGGGCTGATTGATTATCCGGGCCGCGTAATGGCAGAAAAACTGGAGTTTAACGGCCAGGATTTGCAGCGTATCTCAGAA
AAAGAGCGCCGCAACCTGGTGGGTGCCGAAGTGGCGATGATCTTCCAGGACCCGATGACCAGCCTTAACCCGTGCTACAC
CGTGGGTTTCCAGATTATGGAAGCGATTAAGGTGCATCAGGGCGGCAACAAAAGTACCCGCCGTCAGCGAGCGATTGACC
TGCTGAATCAGGTCGGTATTCCCGATCCGGCATCGCGTCTGGATGTTTACCCGCATCAGCTTTCCGGCGGCATGAGCCAG
CGCGTGATGATCGCCATGGCGATTGCCTGTCGGCCAAAACTGCTGATTGCCGATGAACCGACCACCGCGCTGGACGTGAC
CATTCAGGCGCAAATCATCGAACTACTGCTGGAGCTACAGCAGAAAGAGAACATGGCGCTGGTGTTAATTACCCATGACC
TGGCGCTGGTGGCGGAAGCGGCACATAAAATCATCGTGATGTATGCAGGCCAGGTGGTGGAAACCGGTGATGCGCACGCC
ATCTTCCATGCGCCGCGTCACCCGTATACTCAGGCATTGCTGCGTGCGCTGCCAGAATTTGCTCAGGACAAAGAACGTCT
GGCGTCGTTGCCAGGTGTCGTTCCCGGCAAGTACGACCGCCCGAACGGCTGCCTGCTTAACCCGCGCTGCCCCTATGCCA
CTGACAGATGTCGCGCTGAAGAACCGGCGCTGAATATGCTCGCTGACGGGCGTCAGTCCAAATGCCATTACCCACTTGAT
GATGCCGGGAGGCCGACACTATGA

Domains

Predicted by InterProScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (233-297)

• ABC transporter (ABC_tran) (24-181)

3D structure

• AlphaFold DB: A7ZT85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	43.302	98.165	0.425
amiE	Streptococcus thermophilus LMG 18311	42.991	98.165	0.422
amiE	Streptococcus thermophilus LMD-9	42.991	98.165	0.422
oppD	Streptococcus mutans UA159	41.379	97.554	0.404