Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QMY17_RS08865 Genome accession   NZ_CP125812
Coordinates   1683717..1684127 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain N3378-3At     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1660831..1688760 1683717..1684127 within 0


Gene organization within MGE regions


Location: 1660831..1688760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY17_RS08705 - 1660831..1661011 (+) 181 Protein_1696 hypothetical protein -
  QMY17_RS08710 - 1661320..1661550 (+) 231 WP_224588644.1 hypothetical protein -
  QMY17_RS08715 - 1661695..1661943 (+) 249 Protein_1698 hypothetical protein -
  QMY17_RS08720 - 1661906..1662028 (+) 123 Protein_1699 RusA family crossover junction endodeoxyribonuclease -
  QMY17_RS08725 - 1662111..1662263 (+) 153 WP_049832653.1 XtrA/YqaO family protein -
  QMY17_RS08730 - 1662402..1662668 (-) 267 WP_033881358.1 hypothetical protein -
  QMY17_RS08735 - 1663285..1663764 (+) 480 WP_014480344.1 hypothetical protein -
  QMY17_RS08745 - 1664157..1664462 (-) 306 WP_123772463.1 hypothetical protein -
  QMY17_RS08750 terS 1664589..1665153 (+) 565 Protein_1704 phage terminase small subunit -
  QMY17_RS08755 - 1665113..1665588 (+) 476 Protein_1705 phage tail tube protein -
  QMY17_RS08760 - 1665875..1665961 (-) 87 WP_072592549.1 putative holin-like toxin -
  QMY17_RS22470 - 1666136..1666302 (+) 167 Protein_1707 peptidoglycan-binding domain-containing protein -
  QMY17_RS08770 istB 1666554..1667312 (-) 759 WP_014479891.1 IS21-like element helper ATPase IstB -
  QMY17_RS08775 istA 1667309..1668856 (-) 1548 WP_014480339.1 IS21 family transposase -
  QMY17_RS08780 - 1669697..1669987 (-) 291 WP_014480337.1 contact-dependent growth inhibition system immunity protein -
  QMY17_RS08785 atxG 1670097..1670674 (-) 578 Protein_1711 suppressor of fused domain protein -
  QMY17_RS08790 - 1670942..1671175 (-) 234 WP_224588641.1 hypothetical protein -
  QMY17_RS08795 - 1671264..1671467 (+) 204 WP_123772462.1 hypothetical protein -
  QMY17_RS08800 - 1671775..1672254 (-) 480 WP_224588637.1 hypothetical protein -
  QMY17_RS08805 cdiI 1672359..1672718 (-) 360 WP_014480334.1 ribonuclease toxin immunity protein CdiI -
  QMY17_RS08810 - 1672815..1673267 (-) 453 WP_014480333.1 SMI1/KNR4 family protein -
  QMY17_RS08815 - 1673366..1673806 (-) 441 WP_014480332.1 SMI1/KNR4 family protein -
  QMY17_RS08820 - 1674209..1674496 (-) 288 WP_014480331.1 hypothetical protein -
  QMY17_RS22475 - 1674510..1675217 (-) 708 WP_014480330.1 hypothetical protein -
  QMY17_RS22480 - 1675603..1676436 (-) 834 Protein_1720 ribonuclease YeeF family protein -
  QMY17_RS08830 - 1676618..1677745 (+) 1128 WP_014480328.1 Rap family tetratricopeptide repeat protein -
  QMY17_RS08835 - 1678485..1678880 (-) 396 WP_014480327.1 VOC family protein -
  QMY17_RS08840 - 1679822..1680712 (-) 891 WP_014480326.1 LysR family transcriptional regulator -
  QMY17_RS08845 fumC 1680879..1682267 (+) 1389 WP_014480325.1 class II fumarate hydratase -
  QMY17_RS08850 - 1682486..1682698 (+) 213 Protein_1725 recombinase family protein -
  QMY17_RS08855 - 1682695..1682793 (-) 99 WP_031600702.1 hypothetical protein -
  QMY17_RS08860 sigK 1682793..1683521 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  QMY17_RS08865 nucA/comI 1683717..1684127 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  QMY17_RS08870 yqeB 1684160..1684882 (-) 723 WP_014480321.1 hypothetical protein -
  QMY17_RS08875 gnd 1685133..1686026 (+) 894 WP_014480320.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  QMY17_RS08880 yqeD 1686045..1686671 (-) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  QMY17_RS08885 cwlH 1686858..1687610 (+) 753 WP_014480318.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  QMY17_RS08890 yqeF 1687862..1688593 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=832932 QMY17_RS08865 WP_009967785.1 1683717..1684127(+) (nucA/comI) [Bacillus subtilis strain N3378-3At]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=832932 QMY17_RS08865 WP_009967785.1 1683717..1684127(+) (nucA/comI) [Bacillus subtilis strain N3378-3At]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529