Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ULK21_RS01935 Genome accession   NZ_CP139887
Coordinates   417458..418135 (-) Length   225 a.a.
NCBI ID   WP_000178170.1    Uniprot ID   -
Organism   Escherichia coli strain ECO21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412458..423135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ULK21_RS01920 (ULK21_01920) chiA 413816..416518 (+) 2703 WP_001525810.1 bifunctional chitinase/lysozyme -
  ULK21_RS01925 (ULK21_01925) bfd 416687..416881 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  ULK21_RS01930 (ULK21_01930) bfr 416953..417429 (+) 477 WP_000675504.1 bacterioferritin -
  ULK21_RS01935 (ULK21_01935) pilD 417458..418135 (-) 678 WP_000178170.1 prepilin peptidase GspO Machinery gene
  ULK21_RS01940 (ULK21_01940) gspM 418135..418596 (-) 462 WP_001296465.1 type II secretion system protein GspM -
  ULK21_RS01945 (ULK21_01945) gspL 418593..419756 (-) 1164 WP_001527819.1 type II secretion system protein GspL -
  ULK21_RS01950 (ULK21_01950) gspK 419771..420754 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  ULK21_RS01955 (ULK21_01955) gspJ 420747..421334 (-) 588 WP_001527818.1 type II secretion system minor pseudopilin GspJ -
  ULK21_RS01960 (ULK21_01960) gspI 421327..421704 (-) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  ULK21_RS01965 (ULK21_01965) gspH 421701..422210 (-) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  ULK21_RS01970 (ULK21_01970) gspG 422218..422655 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24842.69 Da        Isoelectric Point: 7.6364

>NTDB_id=832589 ULK21_RS01935 WP_000178170.1 417458..418135(-) (pilD) [Escherichia coli strain ECO21]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=832589 ULK21_RS01935 WP_000178170.1 417458..418135(-) (pilD) [Escherichia coli strain ECO21]
ATGACAATGTTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382