Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NSMS1_RS10835 Genome accession   NZ_AP023441
Coordinates   2496497..2497603 (+) Length   368 a.a.
NCBI ID   WP_224093101.1    Uniprot ID   -
Organism   Nostoc sp. MS1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2491497..2502603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSMS1_RS10825 (NSMS1_21300) phnD 2493652..2494611 (-) 960 WP_224093100.1 phosphate/phosphite/phosphonate ABC transporter substrate-binding protein -
  NSMS1_RS10830 (NSMS1_21310) - 2494969..2496267 (-) 1299 WP_224095195.1 ABC transporter substrate-binding protein -
  NSMS1_RS10835 (NSMS1_21320) pilM 2496497..2497603 (+) 1107 WP_224093101.1 type IV pilus assembly protein PilM Machinery gene
  NSMS1_RS10840 (NSMS1_21330) - 2497713..2498480 (+) 768 WP_224093102.1 PilN domain-containing protein -
  NSMS1_RS10845 (NSMS1_21340) - 2498477..2499241 (+) 765 WP_224093103.1 pilus assembly protein PilO -
  NSMS1_RS10850 (NSMS1_21350) - 2499465..2501849 (+) 2385 WP_224093104.1 type IV pilus secretin family protein -
  NSMS1_RS10855 (NSMS1_21360) - 2501993..2502436 (-) 444 WP_224093107.1 cyclic nucleotide-binding domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40555.43 Da        Isoelectric Point: 4.4320

>NTDB_id=83252 NSMS1_RS10835 WP_224093101.1 2496497..2497603(+) (pilM) [Nostoc sp. MS1]
MVKSFSSLFGKSHRGVGVEIAPERVNVVQLRKQRQGLKLESLTSVPVPEGIVSDGQITDPPAMAQLIQQALAESKIKVSS
VATGVPGRDAIVRIIPVPAELDDKELRDMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVATRKEVTDTY
IRTFEQAGLQISVLEINSFALIRTIRDQLRQFGPQEATVLVDIEFDSTEIAIIINGVPQFSRTVPIGTYQMQMAYARAMS
LPTSRDMEILQEMVIPVNSGDGDRTGMTETDPAMAGVLRILGELTDELRRSIDFYLNQSENLEIVQILLGGPGGGLQQLN
EFFTQRLSIPTNQIDPIGSLSLQVDEAKYPSTQRSGLGIVLGLGMREV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=83252 NSMS1_RS10835 WP_224093101.1 2496497..2497603(+) (pilM) [Nostoc sp. MS1]
GTGGTGAAAAGTTTCAGTAGTCTGTTTGGAAAATCTCATAGAGGGGTGGGTGTAGAAATTGCACCAGAACGGGTGAATGT
AGTTCAACTACGCAAGCAGCGCCAAGGCTTGAAACTCGAATCTTTGACATCGGTTCCAGTTCCCGAAGGAATTGTCAGTG
ATGGTCAAATCACCGATCCTCCAGCAATGGCGCAATTAATTCAGCAGGCGCTGGCTGAAAGCAAAATCAAAGTATCTAGT
GTAGCAACTGGTGTCCCTGGAAGGGATGCGATCGTCCGCATCATACCTGTACCAGCAGAGTTGGATGATAAAGAATTACG
GGATATGGTGTTAAACCACGAAGCTGGTTTGTATTTGCCTTATCCCCGTGAGGAAGCTGATGTAGATTATCAAAAACTTG
GGTACTTTGTAGATGAGGACGGCATTGAGAAGGTACAAGTACTTTTAGTTGCTACTCGCAAGGAGGTAACAGATACATAT
ATTAGAACTTTTGAGCAAGCAGGTTTACAAATTAGCGTACTGGAAATCAATAGTTTTGCTTTAATTCGGACAATTCGTGA
TCAACTACGACAATTTGGGCCGCAAGAAGCAACAGTACTAGTTGACATCGAGTTCGACAGTACAGAAATCGCCATCATTA
TTAACGGGGTTCCCCAATTTTCCCGTACTGTCCCCATTGGTACATATCAAATGCAAATGGCTTATGCCAGAGCAATGAGT
TTGCCTACATCACGAGACATGGAAATCTTACAGGAAATGGTAATTCCTGTAAATTCTGGTGATGGTGATAGAACTGGGAT
GACAGAAACCGACCCTGCTATGGCTGGTGTGTTGCGTATCTTGGGAGAACTGACAGATGAGTTACGCCGTTCTATAGATT
TTTACTTAAATCAAAGTGAAAACTTAGAAATCGTCCAGATTCTATTAGGTGGGCCTGGTGGTGGTTTACAGCAACTTAAT
GAGTTTTTTACTCAACGCTTAAGTATTCCCACTAATCAAATAGATCCTATCGGGTCTTTGTCCTTGCAAGTTGATGAAGC
AAAGTATCCCTCTACACAACGTTCTGGTTTAGGAATAGTCCTTGGTTTAGGGATGCGGGAGGTGTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

65.94

99.728

0.658

Multiple sequence alignment