Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   QLH32_RS08025 Genome accession   NZ_CP125669
Coordinates   1687714..1688514 (-) Length   266 a.a.
NCBI ID   WP_267981990.1    Uniprot ID   -
Organism   Acinetobacter sp. KCTC 92772     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1682714..1693514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLH32_RS08005 (QLH32_08005) - 1683775..1684473 (-) 699 WP_283269043.1 tetratricopeptide repeat protein -
  QLH32_RS08010 (QLH32_08010) hisS 1684505..1685797 (-) 1293 WP_283269045.1 histidine--tRNA ligase -
  QLH32_RS08015 (QLH32_08015) ispG 1685794..1686909 (-) 1116 WP_283269046.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  QLH32_RS08020 (QLH32_08020) - 1686932..1687723 (-) 792 WP_283269047.1 helix-turn-helix domain-containing protein -
  QLH32_RS08025 (QLH32_08025) pilF 1687714..1688514 (-) 801 WP_267981990.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  QLH32_RS08030 (QLH32_08030) rlmN 1688532..1689767 (-) 1236 WP_283269048.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  QLH32_RS08035 (QLH32_08035) ndk 1689890..1690321 (-) 432 WP_267981992.1 nucleoside-diphosphate kinase -
  QLH32_RS08040 (QLH32_08040) iscX 1690434..1690634 (-) 201 WP_005269171.1 Fe-S cluster assembly protein IscX -
  QLH32_RS08045 (QLH32_08045) - 1690740..1691291 (-) 552 WP_283269050.1 phosphatase PAP2 family protein -
  QLH32_RS08050 (QLH32_08050) - 1691319..1692605 (-) 1287 WP_283269051.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30132.09 Da        Isoelectric Point: 9.6713

>NTDB_id=832332 QLH32_RS08025 WP_267981990.1 1687714..1688514(-) (pilF) [Acinetobacter sp. KCTC 92772]
MNTTRPKTIIATAVVFTVLGLSACQSTTLKKDPEKGVQVRTQLAAEYIRTGDLDSAKRSLDQALKVDSRDANANMMMGIL
LQQEGSKPNMEKADAYFKRAISSEPNNAQARNNYGTYLYQLQRYNDAIEQFTRAGATLGYDQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNATISMLELAEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=832332 QLH32_RS08025 WP_267981990.1 1687714..1688514(-) (pilF) [Acinetobacter sp. KCTC 92772]
TTGAATACAACTCGACCAAAAACGATTATAGCGACAGCTGTTGTGTTTACTGTATTGGGCCTAAGCGCCTGCCAATCAAC
AACACTGAAGAAAGACCCAGAAAAAGGGGTGCAGGTGCGTACCCAGCTCGCCGCAGAATATATCCGTACAGGTGACTTGG
ATTCAGCCAAACGCTCCCTTGACCAGGCATTGAAAGTCGATTCCCGTGATGCAAACGCCAACATGATGATGGGCATCCTG
CTGCAACAAGAGGGCAGTAAACCCAATATGGAGAAAGCGGACGCCTATTTCAAGCGTGCGATTTCCTCTGAGCCTAATAA
TGCACAGGCCCGTAATAATTACGGTACATATTTGTACCAGCTGCAACGTTATAATGATGCCATTGAGCAATTTACCCGTG
CGGGTGCAACCCTGGGCTATGACCAGCGCTATCAGTCGCTGGAAAATCTGGGGCGGATCTATTTAAAACTGGGCGATGTT
GCGAATGCCGAAAAATCATTCAAACAGGCGCTACAGGCGAATCGAAATGCCACCATCTCCATGTTAGAGTTGGCGGAAAT
TTTCTATTTGCAGCAGAACAATAGAGATGCATCACAGCTTTATGAACAATATGTTCGTAATGTGGGGCAGAAGAATCAGG
GTGCCCGCGCACTCTGGATTGGTATACGCATTGCTCGAGCCAATGCGGATCAGTTGGGAATGCAGGTGTTGGTCAACCAA
ATGCGTGCCTTGTTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(149-207)

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.401

100

0.797
  pilF Acinetobacter baylyi ADP1

71.923

97.744

0.703