Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   QMA40_RS25760 Genome accession   NZ_CP125661
Coordinates   4974080..4975429 (-) Length   449 a.a.
NCBI ID   WP_000879428.1    Uniprot ID   W8YBC3
Organism   Bacillus thuringiensis strain BV5     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4969080..4980429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMA40_RS25725 (QMA40_25660) - 4969124..4969819 (-) 696 WP_000466510.1 hypothetical protein -
  QMA40_RS25730 (QMA40_25665) - 4969806..4970594 (-) 789 WP_000120044.1 hypothetical protein -
  QMA40_RS25735 (QMA40_25670) - 4970622..4971191 (-) 570 WP_001211058.1 hypothetical protein -
  QMA40_RS25740 (QMA40_25675) - 4971309..4971782 (-) 474 WP_000511548.1 hypothetical protein -
  QMA40_RS25745 (QMA40_25680) hpf 4972187..4972729 (-) 543 WP_000671186.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  QMA40_RS25750 (QMA40_25685) cspC 4973052..4973249 (-) 198 WP_001990088.1 cold shock protein CspC -
  QMA40_RS25755 (QMA40_25690) - 4973376..4974080 (-) 705 WP_033685746.1 ComF family protein -
  QMA40_RS25760 (QMA40_25695) comFA 4974080..4975429 (-) 1350 WP_000879428.1 ATP-dependent helicase ComFA Machinery gene
  QMA40_RS25765 (QMA40_25700) - 4975557..4976984 (-) 1428 WP_000499480.1 NlpC/P60 family protein -
  QMA40_RS25770 (QMA40_25705) - 4977133..4977447 (-) 315 WP_000400857.1 winged helix-turn-helix transcriptional regulator -
  QMA40_RS25775 (QMA40_25710) - 4977620..4978462 (-) 843 WP_000684725.1 DegV family protein -
  QMA40_RS25780 (QMA40_25715) - 4978699..4979334 (+) 636 WP_000926665.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51010.89 Da        Isoelectric Point: 9.9712

>NTDB_id=832319 QMA40_RS25760 WP_000879428.1 4974080..4975429(-) (comFA) [Bacillus thuringiensis strain BV5]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=832319 QMA40_RS25760 WP_000879428.1 4974080..4975429(-) (comFA) [Bacillus thuringiensis strain BV5]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGGGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCGCCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCGTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTACATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8YBC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.357

98.441

0.506
  comFA Latilactobacillus sakei subsp. sakei 23K

41.439

89.755

0.372