Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   QMK12_RS05375 Genome accession   NZ_CP125395
Coordinates   1058098..1059048 (+) Length   316 a.a.
NCBI ID   WP_134224604.1    Uniprot ID   -
Organism   Campylobacter jejuni strain SKBC94     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1045081..1069154 1058098..1059048 within 0


Gene organization within MGE regions


Location: 1045081..1069154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK12_RS05310 (QMK12_05310) pglK 1045081..1046775 (-) 1695 WP_134263734.1 ABC-type lipopolysaccharide transporter PglK -
  QMK12_RS05315 (QMK12_05315) galE 1046769..1047755 (-) 987 WP_002891881.1 UDP-glucose 4-epimerase GalE -
  QMK12_RS05320 (QMK12_05320) - 1047814..1048608 (-) 795 WP_283155402.1 3'-5' exonuclease -
  QMK12_RS05325 (QMK12_05325) waaC 1048674..1049702 (+) 1029 WP_088303577.1 lipopolysaccharide heptosyltransferase I -
  QMK12_RS05330 (QMK12_05330) htrB 1049692..1050579 (+) 888 WP_134263733.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  QMK12_RS05335 (QMK12_05335) - 1050576..1052126 (+) 1551 WP_057031452.1 glycosyltransferase -
  QMK12_RS05340 (QMK12_05340) - 1052095..1052982 (-) 888 WP_088303578.1 glycosyltransferase family 2 protein -
  QMK12_RS05345 (QMK12_05345) - 1052975..1054183 (-) 1209 WP_283155403.1 glycosyltransferase family 2 protein -
  QMK12_RS05350 (QMK12_05350) - 1054260..1055237 (-) 978 WP_283155404.1 glycosyltransferase family 2 protein -
  QMK12_RS05355 (QMK12_05355) - 1055295..1055441 (+) 147 WP_166739818.1 glycosyltransferase family 4 protein -
  QMK12_RS05360 (QMK12_05360) - 1055560..1056360 (+) 801 WP_283155405.1 glycosyltransferase -
  QMK12_RS05365 (QMK12_05365) - 1056349..1057179 (-) 831 WP_283155406.1 hypothetical protein -
  QMK12_RS05370 (QMK12_05370) waaV 1057230..1058039 (-) 810 WP_057031457.1 glycosyltransferase family 2 protein -
  QMK12_RS05375 (QMK12_05375) waaF 1058098..1059048 (+) 951 WP_134224604.1 lipopolysaccharide heptosyltransferase II Regulator
  QMK12_RS05380 (QMK12_05380) - 1059034..1059798 (-) 765 WP_240692741.1 glycosyltransferase family 25 protein -
  QMK12_RS05385 (QMK12_05385) gmhA 1059798..1060358 (-) 561 WP_096702627.1 D-sedoheptulose 7-phosphate isomerase -
  QMK12_RS05390 (QMK12_05390) rfaE1 1060355..1061740 (-) 1386 WP_002866169.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  QMK12_RS05395 (QMK12_05395) rfaD 1061733..1062686 (-) 954 WP_070317050.1 ADP-glyceromanno-heptose 6-epimerase -
  QMK12_RS05400 (QMK12_05400) gmhB 1062687..1063247 (-) 561 WP_088303468.1 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  QMK12_RS05405 (QMK12_05405) - 1063328..1063630 (+) 303 WP_070317048.1 cytochrome c -
  QMK12_RS05410 (QMK12_05410) ccoS 1063653..1063859 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -
  QMK12_RS05415 (QMK12_05415) - 1063856..1066213 (-) 2358 WP_088303469.1 heavy metal translocating P-type ATPase -
  QMK12_RS05420 (QMK12_05420) rho 1066323..1067621 (+) 1299 WP_057042622.1 transcription termination factor Rho -
  QMK12_RS05425 (QMK12_05425) dnaX 1067625..1069154 (+) 1530 WP_070317046.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36358.96 Da        Isoelectric Point: 10.2689

>NTDB_id=831867 QMK12_RS05375 WP_134224604.1 1058098..1059048(+) (waaF) [Campylobacter jejuni strain SKBC94]
MKIFIHLPTWLGDAVMASPALYAIKEHFKDAQFILYGSLVSTTLFKEFPNSKIIIENKQSRYKQALSLRKELGKINFSFA
FRSAFSSKIILYILKTKQRYFFNKNKYKEEHQVLKYLYFIENSLDIKAHFKDLKLPFKLKFQNPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNQNAKLVHLDLACMPCMQKTCPLKHHKCMKDLKPEVILKAIQNLINI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=831867 QMK12_RS05375 WP_134224604.1 1058098..1059048(+) (waaF) [Campylobacter jejuni strain SKBC94]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCGCCTGCTTTATATGCTATAAAAGAACA
CTTTAAAGATGCTCAATTTATCCTTTATGGTTCTTTGGTTTCCACAACACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAGCAATCCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAAATCAATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTGTATATTCTTAAAACAAAACAAAGATATTTTTTTAACAAAAACAAGTA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCGCTTGACATAAAAGCTCATTTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAACCCTCTTGTCTTAAAAAATGGTAAAAAAATTCTAGGACTCAACCCTGGCGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAAATTTT
AATCTTTGGTGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACCACTATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTTATCACAAATGACAGT
GGTCCTATGCACATAAGTGCGGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACGAAATTTACCCAAACTTCACC
TTGGCAAAATCAAAACGCAAAATTAGTGCATTTAGATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGTCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTTTAAAAGCAATTCAAAATTTAATTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(102-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.675

99.367

0.921