Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   U0532_RS14530 Genome accession   NZ_CP139808
Coordinates   3014861..3015538 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain FLI21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3009861..3020538
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U0532_RS14515 (U0532_14515) chiA 3011228..3013921 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  U0532_RS14520 (U0532_14520) bfd 3014090..3014284 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  U0532_RS14525 (U0532_14525) bfr 3014356..3014832 (+) 477 WP_000675504.1 bacterioferritin -
  U0532_RS14530 (U0532_14530) pilD 3014861..3015538 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  U0532_RS14535 (U0532_14535) gspM 3015538..3015999 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  U0532_RS14540 (U0532_14540) gspL 3015996..3017159 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  U0532_RS14545 (U0532_14545) gspK 3017174..3018157 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  U0532_RS14550 (U0532_14550) gspJ 3018150..3018737 (-) 588 WP_232586206.1 type II secretion system minor pseudopilin GspJ -
  U0532_RS14555 (U0532_14555) gspI 3018730..3019107 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  U0532_RS14560 (U0532_14560) gspH 3019104..3019613 (-) 510 WP_024238847.1 type II secretion system minor pseudopilin GspH -
  U0532_RS14565 (U0532_14565) gspG 3019621..3020058 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=831677 U0532_RS14530 WP_000178161.1 3014861..3015538(-) (pilD) [Escherichia coli strain FLI21]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=831677 U0532_RS14530 WP_000178161.1 3014861..3015538(-) (pilD) [Escherichia coli strain FLI21]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378