Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   TT393_RS19240 Genome accession   NZ_CP139803
Coordinates   3947020..3947460 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain HN257     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3942020..3952460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TT393_RS19215 (TT393_19215) coaE 3942510..3943130 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  TT393_RS19220 (TT393_19220) - 3943155..3943199 (+) 45 WP_120795372.1 protein YacM -
  TT393_RS19225 (TT393_19225) guaC 3943355..3944398 (+) 1044 WP_001217338.1 GMP reductase -
  TT393_RS19230 (TT393_19230) hofC 3944433..3945635 (-) 1203 WP_000157234.1 protein transport protein HofC -
  TT393_RS19235 (TT393_19235) pilB 3945625..3947010 (-) 1386 WP_001025154.1 type II secretion system protein GspE Machinery gene
  TT393_RS19240 (TT393_19240) pilA 3947020..3947460 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  TT393_RS19245 (TT393_19245) nadC 3947663..3948556 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  TT393_RS19250 (TT393_19250) ampD 3948644..3949195 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  TT393_RS19255 (TT393_19255) ampE 3949192..3950046 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  TT393_RS19260 (TT393_19260) aroP 3950089..3951462 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=831618 TT393_RS19240 WP_000360904.1 3947020..3947460(-) (pilA) [Escherichia coli strain HN257]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=831618 TT393_RS19240 WP_000360904.1 3947020..3947460(-) (pilA) [Escherichia coli strain HN257]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363