Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   QMK47_RS25305 Genome accession   NZ_CP125373
Coordinates   5689741..5691108 (-) Length   455 a.a.
NCBI ID   WP_003185644.1    Uniprot ID   J2YBF9
Organism   Pseudomonas sp. P9_35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5684741..5696108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK47_RS25280 (QMK47_25275) - 5684950..5685669 (+) 720 WP_413817752.1 GntR family transcriptional regulator -
  QMK47_RS25285 (QMK47_25280) yjiA 5685661..5686629 (-) 969 WP_258691275.1 GTPase -
  QMK47_RS25290 (QMK47_25285) - 5686778..5686975 (-) 198 WP_003201656.1 YbdD/YjiX family protein -
  QMK47_RS25295 (QMK47_25290) - 5686991..5689056 (-) 2066 Protein_4996 carbon starvation CstA family protein -
  QMK47_RS25300 (QMK47_25295) - 5689215..5689571 (+) 357 WP_253469541.1 PilZ domain-containing protein -
  QMK47_RS25305 (QMK47_25300) radA 5689741..5691108 (-) 1368 WP_003185644.1 DNA repair protein RadA Machinery gene
  QMK47_RS25310 (QMK47_25305) - 5691150..5691698 (-) 549 WP_320390554.1 ankyrin repeat domain-containing protein -
  QMK47_RS25315 (QMK47_25310) katB 5691851..5693392 (-) 1542 WP_320389477.1 catalase KatB -
  QMK47_RS25320 (QMK47_25315) mscL 5693675..5694088 (+) 414 WP_214512867.1 large-conductance mechanosensitive channel protein MscL -
  QMK47_RS25325 (QMK47_25320) - 5694354..5695130 (-) 777 WP_320389478.1 ferredoxin--NADP reductase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48800.28 Da        Isoelectric Point: 6.8987

>NTDB_id=831491 QMK47_RS25305 WP_003185644.1 5689741..5691108(-) (radA) [Pseudomonas sp. P9_35]
MAKAKRMYGCTECGSTFPKWAGQCSECGAWNTLTETMVESGGAAAPTGRTGWAGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLATRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARLEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=831491 QMK47_RS25305 WP_003185644.1 5689741..5691108(-) (radA) [Pseudomonas sp. P9_35]
ATGGCCAAGGCAAAGCGCATGTACGGCTGCACCGAGTGCGGCTCGACCTTTCCCAAATGGGCCGGCCAGTGCAGCGAATG
CGGGGCCTGGAACACCCTGACTGAAACCATGGTGGAAAGCGGCGGCGCGGCGGCACCCACCGGTCGCACGGGCTGGGCCG
GTCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGTGTCGAAGAAATTCCGCGTTTCTCCACTGCATCCGGAGAACTG
GACCGCGTGCTCGGCGGCGGTTTGGTGGACGGTTCGGTGGTGCTGATCGGCGGCGATCCGGGCATCGGCAAATCCACCAT
CTTGTTGCAAACCTTGTGCAACCTCGCCACCCGCATGCCGGCGCTGTACGTCACTGGCGAAGAATCCCAGCAGCAGGTCG
CCATGCGCGCCCGGCGCCTGGGCTTGCCCCAGGACCAGTTGCGGGTCATGACCGAGACCTGCATCGAGACCATCATCGCC
ACGGCGCGCCTGGAAAAACCCAAGGTGATGGTGATCGACTCGATCCAGACCATTTTCACCGAACAGCTGCAATCGGCCCC
GGGCGGCGTGTCCCAGGTGCGCGAAAGTGCGGCGTTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATTTTCCTGG
TGGGCCACGTCACCAAGGAAGGCGCGCTGGCGGGGCCACGCGTGCTGGAGCACATGGTCGATACCGTGTTGTATTTCGAA
GGCGAATCCGATGGTCGCCTGCGTTTGCTGCGGGCGGTGAAGAACCGCTTTGGTGCCGTGAACGAACTGGGTGTGTTCGG
CATGACCGACAGAGGCTTGAAAGAAGTCTCAAACCCTTCGGCGATCTTTCTGACGCGTGCCCAGGAAGAAGTCCCTGGTA
GCGTGGTCATGGCAACGTGGGAAGGCACCCGGCCGATGCTGGTCGAAGTGCAAGCGCTGGTGGACGACAGTCACCTGGCG
AACCCGCGCCGGGTCACCCTGGGCCTGGATCAGAATCGGTTGGCCATGTTGCTGGCGGTGCTGCATCGACACGGCGGGAT
TCCGACGCACGACCAGGACGTGTTCCTCAACGTCGTGGGCGGGGTCAAGGTGCTGGAAACCGCGTCCGACCTGGCGTTGA
TGGCCGCAGTCATGTCCAGCCTGCGCAACCGGCCGCTGCCCCACGACCTGTTGGTGTTCGGCGAAGTGGGGCTGTCGGGC
GAAGTACGGCCGGTGCCCAGTGGCCAGGAGCGACTCAAGGAAGCGGCCAAGCACGGTTTCAAACGCGCCATCGTGCCCAA
GGGCAACGCGCCGAAGGAAGCCCCGCCGGGGTTGCAGATCATTGCCGTGACGCGCCTGGAACAGGCGTTGGATGCGTTGT
TCGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(80-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J2YBF9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.015

100

0.492
  radA Streptococcus pneumoniae Rx1

46.304

100

0.468
  radA Streptococcus pneumoniae R6

46.304

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.304

100

0.468
  radA Streptococcus pneumoniae D39

46.304

100

0.468
  radA Streptococcus mitis SK321

46.491

100

0.466
  radA Streptococcus mitis NCTC 12261

46.272

100

0.464