Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   QMK47_RS25070 Genome accession   NZ_CP125373
Coordinates   5637804..5639393 (+) Length   529 a.a.
NCBI ID   WP_320389471.1    Uniprot ID   -
Organism   Pseudomonas sp. P9_35     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5632804..5644393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK47_RS25050 (QMK47_25045) pgeF 5634605..5635330 (-) 726 WP_320389468.1 peptidoglycan editing factor PgeF -
  QMK47_RS25055 (QMK47_25050) rluD 5635327..5636289 (-) 963 WP_320389469.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  QMK47_RS25060 (QMK47_25055) - 5636440..5637456 (+) 1017 WP_320389470.1 outer membrane protein assembly factor BamD -
  QMK47_RS25065 (QMK47_25060) - 5637581..5637814 (+) 234 WP_014340265.1 PP0621 family protein -
  QMK47_RS25070 (QMK47_25065) pilS 5637804..5639393 (+) 1590 WP_320389471.1 two-component system sensor histidine kinase NtrB Regulator
  QMK47_RS25075 (QMK47_25070) pilR 5639390..5640736 (+) 1347 WP_253469484.1 sigma-54-dependent transcriptional regulator Regulator
  QMK47_RS25080 (QMK47_25075) - 5640828..5641193 (-) 366 WP_253469487.1 DUF6124 family protein -
  QMK47_RS25085 (QMK47_25080) - 5641505..5641693 (+) 189 WP_253478679.1 nucleoid-structuring protein H-NS -
  QMK47_RS25090 (QMK47_25085) - 5642015..5642179 (-) 165 WP_253478682.1 hypothetical protein -
  QMK47_RS25095 (QMK47_25090) thiO 5642413..5643513 (-) 1101 WP_258689405.1 glycine oxidase ThiO -
  QMK47_RS25100 (QMK47_25095) - 5643570..5643971 (-) 402 WP_018600956.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58876.57 Da        Isoelectric Point: 7.1770

>NTDB_id=831489 QMK47_RS25070 WP_320389471.1 5637804..5639393(+) (pilS) [Pseudomonas sp. P9_35]
MSAEASSPREKQAQRLLRLYHLYRLSIGITLVLLISSKLDNRLLEFANDDLLRSGSWLYLVLNILLVVFLENTRRPARLF
GLALTDVLLLSWLFFVAGGVPSAIGNLLIVSVAIGNTLLRGRIGLLIAAVATLGIVGSSFFLGLSDSNRPSSYLQAGTLG
ALCFAAALLVQGLTRRLEASETLAEQRASEVIGLEALNALILQRMRTGILVLDRERRVQLANESALNLLGMHDLAGQQLD
DYSTALVERMQLWRNNPSLRPSSLTVTGTGLTLQPSFIALGHQDQQQILVFLEDLAQVAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNDADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTTPQRLDLRTWLDQFVRQARESMAEH
QHLHLSIDPGDYTTLMDPDQLTQVLDNLLRNAWRHSAMAHEQAEAWLKLFIDPHSQLSTLDIIDNGPGVTPEQQAHLFEP
FFTTSSQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQI

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=831489 QMK47_RS25070 WP_320389471.1 5637804..5639393(+) (pilS) [Pseudomonas sp. P9_35]
GTGAGCGCTGAGGCCTCAAGCCCACGCGAAAAACAGGCGCAGCGCCTGCTGCGTCTCTATCACCTCTATCGTCTGAGCAT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAAACTGGACAACCGCCTGCTGGAGTTCGCCAATGACGACCTGCTGCGCA
GTGGCAGTTGGTTGTACCTGGTGCTGAATATCCTGCTGGTGGTGTTCCTGGAGAACACCCGCCGCCCTGCCCGGCTGTTC
GGCCTGGCCCTGACTGACGTGCTGCTGCTCTCGTGGCTGTTCTTCGTCGCGGGCGGTGTGCCCAGTGCCATCGGCAACCT
GCTCATTGTCTCGGTGGCCATCGGCAACACGCTGCTGCGAGGCCGCATCGGCCTGTTGATCGCGGCCGTCGCCACCCTTG
GCATCGTCGGTTCGAGTTTCTTTCTTGGCTTGAGCGACTCGAACCGTCCCAGCAGTTATTTGCAGGCTGGCACCCTCGGG
GCACTGTGCTTTGCCGCCGCGCTGTTGGTGCAAGGCCTGACCCGACGCTTGGAAGCCAGCGAAACCCTGGCCGAGCAACG
GGCCAGCGAGGTGATAGGCCTGGAAGCGCTCAACGCCCTGATTCTGCAACGCATGCGCACCGGCATCCTGGTGCTTGACC
GTGAGCGTCGGGTGCAATTGGCCAACGAAAGCGCGCTGAACCTGCTGGGCATGCACGACCTGGCGGGCCAGCAGCTCGAC
GATTACTCCACCGCCCTGGTCGAACGCATGCAATTGTGGCGAAACAACCCCAGCCTGCGCCCTTCCAGCCTGACCGTTAC
CGGCACGGGCCTGACCCTGCAACCGAGCTTCATCGCCTTGGGGCATCAAGATCAGCAGCAGATCCTGGTGTTCCTTGAAG
ACCTGGCCCAGGTTGCCCAGCAGGCCCAGCAGTTGAAACTCGCTTCCCTCGGGCGCCTCACTGCTGGCATCGCCCATGAA
ATCCGCAACCCCTTGGGCGCCATCAGCCACGCGGCGCAATTACTGCGCGAATCCGAGGAACTGAACGACGCGGATCGGCG
TCTGACGCAGATTATTCAAGATCACTCCCAACGAATGAACCGCGTCATTGAAAACGTCCTGCAGCTGTCTCGCCGCCAGC
AAACCACCCCCCAACGCCTCGACCTGCGGACCTGGCTCGACCAGTTCGTCCGGCAGGCCCGCGAAAGCATGGCCGAGCAC
CAGCACTTGCACCTGAGCATCGACCCGGGTGACTACACCACGCTGATGGACCCCGACCAGTTGACCCAGGTACTCGACAA
CCTGCTGCGCAACGCCTGGCGCCACAGCGCCATGGCCCACGAACAGGCCGAGGCCTGGCTGAAACTGTTCATCGACCCCC
ACAGCCAGCTGTCCACGCTGGACATCATCGACAACGGCCCCGGTGTAACCCCCGAGCAGCAGGCGCATTTGTTCGAACCC
TTCTTTACCACCAGCAGCCAGGGCACCGGCCTTGGGCTCTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGTTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAGATTTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

60.985

99.811

0.609