Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KI236_RS03075 Genome accession   NZ_AP023415
Coordinates   631086..632456 (-) Length   456 a.a.
NCBI ID   WP_212819216.1    Uniprot ID   A0A810PWF1
Organism   Vescimonas fastidiosa strain MM35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 626086..637456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI236_RS03055 (MM35RIKEN_06200) - 626697..627713 (-) 1017 WP_329958962.1 tyrosine recombinase XerC -
  KI236_RS03060 (MM35RIKEN_06210) - 628281..629609 (+) 1329 WP_212819210.1 amidase domain-containing protein -
  KI236_RS03065 (MM35RIKEN_06220) - 629685..630590 (-) 906 WP_212819212.1 phosphate acyltransferase -
  KI236_RS03070 (MM35RIKEN_06230) - 630752..631045 (+) 294 WP_212819214.1 hypothetical protein -
  KI236_RS03075 (MM35RIKEN_06240) radA 631086..632456 (-) 1371 WP_212819216.1 DNA repair protein RadA Machinery gene
  KI236_RS03080 (MM35RIKEN_06250) - 632459..633178 (-) 720 WP_212819218.1 N-acetylmuramoyl-L-alanine amidase -
  KI236_RS03085 (MM35RIKEN_06260) - 633332..633802 (+) 471 WP_228738079.1 spore coat protein CotJB -
  KI236_RS03090 (MM35RIKEN_06270) - 633802..634398 (+) 597 WP_212819220.1 manganese catalase family protein -
  KI236_RS03095 (MM35RIKEN_06280) - 634456..635256 (-) 801 WP_212819222.1 MerR family transcriptional regulator -
  KI236_RS03105 (MM35RIKEN_06290) cas7c 636231..637121 (-) 891 WP_212819224.1 type I-C CRISPR-associated protein Cas7/Csd2 -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49355.89 Da        Isoelectric Point: 6.4918

>NTDB_id=83133 KI236_RS03075 WP_212819216.1 631086..632456(-) (radA) [Vescimonas fastidiosa strain MM35]
MKAKTLFYCTQCGNETPKWAGRCPSCGAWNTIVEQPDIRASVKKGTRSSIKGPVKASAITDMSTDQEIRFSTGMGELDRV
LGGGAVKGSLVLVGGAPGIGKSTLMLQICKRLGEQFRVLYVSGEESVHQLKLRAQRLHAESENLFVLSETNLGDVLECVQ
TEQPDILIVDSIQTLYNEEINSPAGGIGQVKDCTMKLMQLAKEGNITIFVIGHVNKEGFIAGPKVLEHMVDCVLYFEGDQ
HMTYRILRAAKNRFGATNEIGVFEMLDSGLREVENPSEMLLSGRPEDAPGSCVTCVMEGARPVLAEVQALVAPSNANYPR
RTCNGLDSNRAAMLLAVLEKRGGLKVSQCDAYFNIIGGLTLDEPASDLAVLTAIASSYLDKPVPTKLAAVGEVGLSGELR
SINHIEQRLAEVQRLGFTQCIVPSQQAHKLRAPGDLRLLPVKNIGQVLRLLAHDDL

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=83133 KI236_RS03075 WP_212819216.1 631086..632456(-) (radA) [Vescimonas fastidiosa strain MM35]
ATGAAAGCCAAAACTCTATTCTATTGTACCCAGTGCGGCAACGAAACACCTAAGTGGGCGGGGCGCTGCCCTTCCTGCGG
CGCCTGGAACACCATCGTGGAGCAGCCGGATATAAGGGCGTCTGTTAAAAAGGGCACTCGTTCATCTATTAAAGGACCTG
TAAAGGCAAGTGCCATTACAGATATGTCCACCGATCAGGAGATTCGATTTTCCACCGGTATGGGTGAGCTGGATCGGGTT
TTAGGCGGCGGCGCGGTGAAAGGCTCTCTAGTGCTGGTGGGTGGAGCTCCCGGCATCGGTAAGTCCACCCTCATGCTGCA
AATCTGTAAAAGACTGGGTGAGCAGTTCCGGGTTCTCTATGTCTCCGGCGAAGAATCCGTCCACCAACTCAAGCTCCGGG
CGCAGCGTTTGCACGCAGAAAGTGAAAATCTTTTCGTCCTCTCGGAGACGAATTTGGGCGATGTGCTGGAGTGCGTTCAG
ACCGAACAGCCGGACATTCTCATTGTGGACTCCATTCAGACTCTGTATAACGAGGAGATCAATTCTCCCGCCGGCGGTAT
CGGTCAGGTTAAGGACTGCACCATGAAGCTTATGCAGCTGGCTAAAGAGGGAAATATCACCATTTTTGTCATCGGCCATG
TGAATAAAGAGGGCTTTATAGCCGGGCCAAAGGTCCTGGAACACATGGTGGACTGTGTGCTCTACTTTGAGGGCGATCAG
CACATGACTTACCGCATCCTGCGCGCCGCCAAAAACCGCTTCGGAGCTACCAACGAGATCGGCGTATTTGAGATGCTGGA
CAGTGGTCTGCGGGAGGTAGAGAATCCCTCTGAGATGCTTCTCTCCGGCCGTCCGGAGGATGCCCCCGGCTCCTGCGTTA
CCTGTGTGATGGAGGGTGCCCGCCCGGTCCTGGCCGAGGTGCAGGCCCTAGTAGCCCCGTCCAACGCCAATTATCCCCGC
CGCACCTGCAATGGTCTGGATTCTAACCGCGCCGCCATGCTCTTGGCGGTGCTGGAAAAGCGCGGCGGCCTGAAGGTCTC
CCAGTGCGACGCCTATTTTAACATCATCGGCGGCCTGACTCTGGACGAGCCCGCCTCCGATCTGGCCGTTCTGACCGCCA
TTGCCTCCAGCTATTTGGATAAGCCTGTCCCCACCAAGCTGGCCGCCGTAGGGGAGGTGGGCCTCTCCGGTGAACTGCGC
AGCATCAACCATATTGAGCAGCGTTTAGCCGAGGTGCAGCGCCTGGGCTTCACCCAGTGTATCGTCCCGTCCCAGCAGGC
CCATAAGCTCCGCGCTCCCGGTGACCTGCGCCTCCTACCCGTTAAAAACATCGGACAAGTCCTGCGCCTTCTGGCTCACG
ACGACCTTTAA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(361-450)

AAA domain (AAA_25)

(77-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(7-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A810PWF1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae D39

51.106

99.123

0.507
  radA Streptococcus mitis NCTC 12261

51.106

99.123

0.507
  radA Streptococcus pneumoniae Rx1

51.106

99.123

0.507
  radA Streptococcus mitis SK321

51.106

99.123

0.507
  radA Streptococcus pneumoniae R6

51.106

99.123

0.507
  radA Streptococcus pneumoniae TIGR4

51.106

99.123

0.507
  radA Bacillus subtilis subsp. subtilis str. 168

50.554

98.904

0.5

Multiple sequence alignment