Detailed information NTG0831235

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: R6I59_RS08725
• Genome accession: NZ_CP139744
• Coordinates: 1669734..1670723 (+)
• Length: 329 a.a.
• NCBI ID: WP_003232026.1
• Uniprot ID: P51835
• Organism: Bacillus subtilis isolate FELIX_MS255
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1664734..1675723

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6I59_RS08710 (R6I59_08710)	acpP	1664931..1665164 (+)	234	WP_003154310.1	acyl carrier protein	-
R6I59_RS08715 (R6I59_08715)	rncS	1665304..1666053 (+)	750	WP_003232030.1	ribonuclease III	-
R6I59_RS08720 (R6I59_08720)	smc	1666154..1669714 (+)	3561	WP_003232028.1	chromosome segregation protein SMC	-
R6I59_RS08725 (R6I59_08725)	pilA	1669734..1670723 (+)	990	WP_003232026.1	signal recognition particle-docking protein FtsY	Machinery gene
R6I59_RS08730 (R6I59_08730)	sivC	1670760..1671245 (-)	486	WP_003245834.1	Ig-like domain-containing protein	-
R6I59_RS08735 (R6I59_08735)	srpM	1671422..1671754 (+)	333	WP_003238590.1	putative DNA-binding protein	-
R6I59_RS08740 (R6I59_08740)	ffh	1671768..1673108 (+)	1341	WP_003232020.1	signal recognition particle protein	-
R6I59_RS08745 (R6I59_08745)	rpsP	1673214..1673486 (+)	273	WP_003220815.1	30S ribosomal protein S16	-
R6I59_RS08750 (R6I59_08750)	khpA	1673486..1673731 (+)	246	WP_003232017.1	KH domain-containing protein	-
R6I59_RS08755 (R6I59_08755)	ylqD	1673853..1674239 (+)	387	WP_003232015.1	YlqD family protein	-
R6I59_RS08760 (R6I59_08760)	rimM	1674244..1674768 (+)	525	WP_003232013.1	ribosome maturation factor RimM	-
R6I59_RS08765 (R6I59_08765)	trmD	1674765..1675496 (+)	732	WP_003232011.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-

Sequence

Protein

• Length: 329 a.a.
• Molecular weight: 36342.52 Da
• Isoelectric Point: 5.0164

>NTDB_id=831235 R6I59_RS08725 WP_003232026.1 1669734..1670723(+) (pilA) [Bacillus subtilis isolate FELIX_MS255]
MSFFKKLKEKITKQTDSVSEKFKDGLEKTRNSFQNKVNDLVSRYRKVDEDFFEELEEVLISADVGFTTVMELIDELKKEV
KRRNIQDPKEVQSVISEKLVEIYNSGDEQISELNIQDGRLNVILLVGVNGVGKTTTIGKLAHKMKQEGKSVVLAAGDTFR
AGAIEQLEVWGERTGVPVIKQTAGSDPAAVIYDAVHAAKARNADVLICDTAGRLQNKVNLMKELEKVKRVIEREVPEAPH
EVLLALDATTGQNAMAQAKEFSKATNVTGIALTKLDGTAKGGIVLAIRNELHIPVKLVGLGEKVDDLQEFDPESYVYGLF
SDLVEKADD

Nucleotide

• Length: 990 bp

>NTDB_id=831235 R6I59_RS08725 WP_003232026.1 1669734..1670723(+) (pilA) [Bacillus subtilis isolate FELIX_MS255]
ATGAGCTTTTTTAAAAAATTAAAAGAGAAAATCACAAAACAGACAGATTCCGTATCTGAAAAGTTTAAGGATGGCCTTGA
AAAAACAAGAAACTCCTTTCAAAACAAAGTGAATGATCTTGTATCCCGTTACCGTAAAGTGGATGAGGATTTCTTCGAAG
AGCTTGAAGAGGTTCTTATCAGCGCGGATGTCGGTTTTACAACCGTTATGGAATTAATAGATGAGCTGAAAAAAGAAGTC
AAACGCAGAAATATTCAAGATCCAAAGGAAGTCCAGTCAGTGATTTCTGAGAAACTGGTCGAGATTTATAACAGCGGAGA
TGAGCAAATTTCAGAACTGAACATCCAGGATGGGCGTTTAAACGTAATCCTTCTGGTAGGTGTAAACGGCGTCGGGAAAA
CAACAACGATCGGAAAGCTTGCTCATAAAATGAAACAAGAAGGAAAATCTGTTGTACTTGCCGCCGGAGACACTTTTAGA
GCGGGAGCCATTGAACAGCTGGAAGTATGGGGAGAGCGTACAGGAGTGCCTGTCATTAAGCAGACGGCAGGAAGCGATCC
GGCGGCTGTCATCTACGATGCTGTTCATGCTGCGAAAGCAAGAAATGCCGATGTATTAATTTGTGATACGGCAGGGCGTC
TCCAAAACAAAGTAAATCTCATGAAAGAGCTTGAAAAAGTAAAACGTGTTATCGAAAGAGAAGTTCCTGAAGCTCCGCAT
GAGGTGCTGCTTGCCCTTGATGCCACGACCGGCCAAAATGCAATGGCTCAGGCAAAAGAATTCTCTAAAGCAACAAATGT
TACCGGCATTGCTTTAACGAAGCTTGACGGTACGGCAAAAGGCGGTATCGTCCTTGCGATTCGCAACGAGCTTCACATCC
CGGTTAAACTAGTCGGTTTAGGAGAAAAAGTTGATGACCTTCAGGAATTTGATCCAGAATCCTATGTGTACGGACTCTTT
TCAGATTTAGTGGAAAAAGCCGACGATTAA

Domains

Predicted by InterProScan.

• SRP54-type protein, GTPase domain (SRP54) (121-320)

• SRP54-type protein, helical bundle domain (SRP54_N) (34-99)

3D structure

• AlphaFold DB: P51835

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	43.709	91.793	0.401