Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QL281_RS14520 Genome accession   NZ_CP125292
Coordinates   2684828..2685277 (-) Length   149 a.a.
NCBI ID   WP_014475770.1    Uniprot ID   A0A0D1KPR3
Organism   Bacillus subtilis strain SRCM117797     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2679828..2690277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QL281_RS14485 (QL281_14485) yckA 2680103..2680783 (-) 681 WP_242304515.1 amino acid ABC transporter permease -
  QL281_RS14490 (QL281_14490) yckB 2680793..2681656 (-) 864 WP_237983781.1 transporter substrate-binding domain-containing protein -
  QL281_RS14495 (QL281_14495) - 2681819..2682009 (+) 191 Protein_2817 hypothetical protein -
  QL281_RS14500 (QL281_14500) - 2682054..2682365 (+) 312 Protein_2818 RDD family protein -
  QL281_RS14505 (QL281_14505) yckD 2682447..2682779 (+) 333 WP_015252849.1 YckD family protein -
  QL281_RS14510 (QL281_14510) bglC 2682933..2684366 (+) 1434 WP_017696125.1 6-phospho-beta-glucosidase -
  QL281_RS14515 (QL281_14515) nin/comJ 2684403..2684801 (-) 399 WP_017696126.1 DNA-entry nuclease inhibitor Regulator
  QL281_RS14520 (QL281_14520) nucA/comI 2684828..2685277 (-) 450 WP_014475770.1 DNA-entry nuclease Machinery gene
  QL281_RS14525 (QL281_14525) tlpC 2685445..2687166 (-) 1722 Protein_2823 methyl-accepting chemotaxis protein TlpC -
  QL281_RS14530 (QL281_14530) hxlB 2687277..2687834 (-) 558 WP_017696129.1 6-phospho-3-hexuloisomerase -
  QL281_RS14535 (QL281_14535) hxlA 2687840..2688472 (-) 633 WP_017696130.1 3-hexulose-6-phosphate synthase -
  QL281_RS14540 (QL281_14540) hxlR 2688704..2689066 (+) 363 WP_017696131.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16440.39 Da        Isoelectric Point: 4.5282

>NTDB_id=830917 QL281_RS14520 WP_014475770.1 2684828..2685277(-) (nucA/comI) [Bacillus subtilis strain SRCM117797]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETIAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=830917 QL281_RS14520 WP_014475770.1 2684828..2685277(-) (nucA/comI) [Bacillus subtilis strain SRCM117797]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(52-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1KPR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

98.658

100

0.987