Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R3F75_RS41515 Genome accession   NZ_CP139649
Coordinates   8309802..8311256 (-) Length   484 a.a.
NCBI ID   WP_011084688.1    Uniprot ID   P30332
Organism   Bradyrhizobium japonicum strain CNPSo 34     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 8304802..8316256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3F75_RS41480 (R3F75_41470) - 8304932..8306317 (-) 1386 WP_011084694.1 ABC transporter substrate-binding protein -
  R3F75_RS41485 (R3F75_41475) - 8306677..8306895 (+) 219 WP_161966258.1 hypothetical protein -
  R3F75_RS41490 (R3F75_41480) - 8306940..8307195 (-) 256 Protein_8001 IS5/IS1182 family transposase -
  R3F75_RS41495 (R3F75_41485) - 8307275..8307637 (-) 363 WP_026312353.1 Wadjet anti-phage system protein JetD domain-containing protein -
  R3F75_RS41500 (R3F75_41490) - 8307634..8307978 (-) 345 WP_085973967.1 hypothetical protein -
  R3F75_RS41505 (R3F75_41495) - 8308536..8308706 (-) 171 WP_014497906.1 hypothetical protein -
  R3F75_RS41510 (R3F75_41500) - 8308743..8309117 (+) 375 WP_269465757.1 di-heme-cytochrome C peroxidase -
  R3F75_RS41515 (R3F75_41505) rpoN/rpoN1 8309802..8311256 (-) 1455 WP_011084688.1 RNA polymerase factor sigma-54 Machinery gene
  R3F75_RS41525 (R3F75_41515) - 8313550..8314335 (-) 786 WP_018645272.1 helix-turn-helix transcriptional regulator -
  R3F75_RS41530 (R3F75_41520) - 8315435..8316049 (-) 615 WP_011084684.1 J domain-containing protein -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 53772.80 Da        Isoelectric Point: 4.7595

>NTDB_id=830670 R3F75_RS41515 WP_011084688.1 8309802..8311256(-) (rpoN/rpoN1) [Bradyrhizobium japonicum strain CNPSo 34]
MALTQRLEFRQSQSLVMSPQLMQAIKLLQLSNLDLMTFVEEELECNPLLERASDDAAGAEAPTEVDQVSGDQLAEAQVRD
ARDGAMTTYTEWGGGGSGDEDYNLEAFVASETTLSDHLAEQLSVAFTAPAQRMIGQYLIDLVDEAGYLPPDLGQAAERLG
ATQEDVEHVLAVLQEFDPPGVCARNLRECLAIQLRELDRYDPAMQALVEHLDLLAKRDIASLRKLCGVDDEDIADMIDEL
RRLSPKPGMKFGSARLQTMVPDVYVRPAPDGGWHVELNSDTLPRVLVNQTYYSKLSKKIGKDVDKSYFNDALQNATWLVR
ALDQRARTILKVATEIVRQQDGFFTLGVAHLRPLNLKAVAEAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIPSAD
GGEAHSAEAVRHRIKQLIESEEPSAVLSDDAIVERLRVSGIDIARRTVAKYREAMRIRSSVQRRRDNMWSTMNSRASGGT
GLDK

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=830670 R3F75_RS41515 WP_011084688.1 8309802..8311256(-) (rpoN/rpoN1) [Bradyrhizobium japonicum strain CNPSo 34]
ATGGCGCTCACGCAAAGATTAGAGTTCCGGCAATCCCAGTCGCTGGTCATGTCGCCGCAGCTGATGCAGGCGATCAAGCT
GCTGCAATTATCTAATCTCGACCTCATGACCTTCGTGGAGGAAGAGCTGGAGTGTAATCCGCTGCTCGAGCGTGCCAGTG
ACGATGCAGCTGGGGCCGAAGCCCCGACTGAGGTCGATCAGGTCAGCGGCGATCAGCTGGCCGAGGCCCAAGTGCGCGAC
GCCCGGGATGGCGCCATGACCACCTATACCGAATGGGGTGGCGGCGGCTCGGGTGACGAAGACTACAATCTCGAAGCGTT
TGTCGCGTCCGAGACAACATTGTCCGACCACCTGGCCGAACAACTGTCGGTCGCATTCACCGCGCCGGCGCAGCGCATGA
TCGGGCAGTATCTGATCGATCTCGTCGACGAAGCCGGCTATCTGCCGCCGGATCTCGGCCAGGCCGCCGAGCGCCTCGGC
GCAACGCAGGAGGATGTCGAGCACGTTCTTGCCGTCCTGCAGGAGTTCGATCCGCCCGGCGTCTGTGCGCGTAACTTGCG
CGAGTGCCTGGCGATCCAGCTCCGCGAGCTCGATAGATACGATCCGGCGATGCAGGCCCTCGTCGAGCATCTCGATCTCC
TCGCCAAGCGCGACATCGCGAGCTTGCGCAAGCTCTGCGGCGTCGACGACGAGGACATCGCCGACATGATCGACGAGCTC
CGCCGGCTCAGTCCCAAGCCGGGCATGAAGTTCGGGTCGGCGCGGCTGCAGACGATGGTACCCGACGTCTATGTCCGTCC
GGCTCCTGATGGCGGCTGGCATGTCGAGCTCAACAGCGACACCTTGCCGCGCGTGCTGGTCAACCAGACCTATTATTCCA
AGCTGTCGAAGAAGATCGGCAAGGACGTCGATAAGTCCTACTTCAACGACGCGCTGCAGAACGCGACCTGGCTGGTGCGC
GCGCTCGACCAGCGCGCCCGCACCATCCTGAAAGTTGCGACCGAGATCGTGCGTCAGCAGGACGGCTTCTTTACCCTTGG
TGTTGCGCATTTGCGGCCGCTGAATCTAAAGGCCGTTGCCGAGGCCATCCAGATGCATGAATCCACGGTGTCGCGCGTCA
CCGCCAACAAATACATGGCAACAAATCGCGGCACATTCGAGTTGAAATATTTCTTCACGGCATCGATCCCTTCGGCGGAT
GGCGGTGAGGCGCATTCCGCTGAAGCGGTGCGTCACCGCATCAAGCAGCTGATCGAATCCGAAGAGCCGTCAGCGGTTCT
GTCCGATGACGCGATCGTTGAGCGCCTGCGAGTCTCGGGCATTGATATTGCCCGCCGCACGGTCGCGAAGTACCGCGAAG
CCATGCGCATTCGGTCCTCGGTGCAGCGCCGCCGCGACAATATGTGGTCAACGATGAACAGTAGAGCATCGGGCGGAACT
GGCCTCGATAAATAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(6-50)

Sigma-54, DNA binding domain (Sigma54_DBD)

(306-465)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(104-291)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P30332

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

38.337

100

0.39