Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OH685_RS05295 Genome accession   NZ_CP125227
Coordinates   1092763..1093563 (+) Length   266 a.a.
NCBI ID   WP_002114699.1    Uniprot ID   -
Organism   Acinetobacter pittii strain SO1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1087763..1098563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OH685_RS05270 (OH685_05320) - 1088679..1089968 (+) 1290 WP_282464130.1 glycosyltransferase family 1 protein -
  OH685_RS05275 (OH685_05325) - 1089993..1090544 (+) 552 WP_005066176.1 phosphatase PAP2 family protein -
  OH685_RS05280 (OH685_05330) iscX 1090644..1090844 (+) 201 WP_069036256.1 Fe-S cluster assembly protein IscX -
  OH685_RS05285 (OH685_05335) ndk 1090958..1091389 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  OH685_RS05290 (OH685_05340) rlmN 1091513..1092748 (+) 1236 WP_002115097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  OH685_RS05295 (OH685_05345) pilF 1092763..1093563 (+) 801 WP_002114699.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OH685_RS05300 (OH685_05350) - 1093554..1094345 (+) 792 WP_069141760.1 helix-turn-helix domain-containing protein -
  OH685_RS05305 (OH685_05355) ispG 1094367..1095482 (+) 1116 WP_002115078.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  OH685_RS05310 (OH685_05360) hisS 1095479..1096771 (+) 1293 WP_039764397.1 histidine--tRNA ligase -
  OH685_RS05315 (OH685_05365) - 1096800..1097498 (+) 699 WP_017398816.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30114.38 Da        Isoelectric Point: 9.0417

>NTDB_id=830448 OH685_RS05295 WP_002114699.1 1092763..1093563(+) (pilF) [Acinetobacter pittii strain SO1]
MSIPKLKTVLCMGIAVAFLVSGCQTTHTQKKDPEKAVKVRTQLAAEHIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=830448 OH685_RS05295 WP_002114699.1 1092763..1093563(+) (pilF) [Acinetobacter pittii strain SO1]
TTGAGTATTCCAAAGTTAAAAACGGTTTTATGTATGGGGATTGCAGTTGCATTTCTGGTAAGTGGTTGCCAGACAACGCA
TACGCAAAAAAAAGACCCTGAGAAAGCGGTAAAGGTGCGTACACAACTTGCTGCTGAACATATCCGTTCGGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTTAAAAGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCTTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_8)

(39-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

93.985

100

0.94
  pilF Acinetobacter baylyi ADP1

76.154

97.744

0.744