Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SM146_RS02230 Genome accession   NZ_CP139360
Coordinates   482439..483116 (-) Length   225 a.a.
NCBI ID   WP_001377644.1    Uniprot ID   -
Organism   Escherichia coli strain EC147566     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477439..488116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM146_RS02215 (SM146_02215) chiA 478806..481499 (+) 2694 WP_001377643.1 bifunctional chitinase/lysozyme -
  SM146_RS02220 (SM146_02220) bfd 481668..481862 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SM146_RS02225 (SM146_02225) bfr 481934..482410 (+) 477 WP_000675504.1 bacterioferritin -
  SM146_RS02230 (SM146_02230) pilD 482439..483116 (-) 678 WP_001377644.1 prepilin peptidase GspO Machinery gene
  SM146_RS02235 (SM146_02235) gspM 483116..483577 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  SM146_RS02240 (SM146_02240) gspL 483574..484737 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  SM146_RS02245 (SM146_02245) - 484752..484955 (-) 204 Protein_445 type II secretion system protein K -
  SM146_RS02250 (SM146_02250) istA 485042..486064 (+) 1023 WP_000255956.1 IS21-like element IS100 family transposase -
  SM146_RS02255 (SM146_02255) istB 486061..486843 (+) 783 WP_001317493.1 IS21-like element IS100kyp family helper ATPase IstB -
  SM146_RS02260 (SM146_02260) gspK 486910..487695 (-) 786 Protein_448 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.75 Da        Isoelectric Point: 7.8986

>NTDB_id=830223 SM146_RS02230 WP_001377644.1 482439..483116(-) (pilD) [Escherichia coli strain EC147566]
MTMLLPLFILVGFIAGCFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=830223 SM146_RS02230 WP_001377644.1 482439..483116(-) (pilD) [Escherichia coli strain EC147566]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTGTTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382
  pilD Vibrio cholerae strain A1552

35.745

100

0.373