Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SIY75_RS18330 Genome accession   NZ_CP139339
Coordinates   3765696..3766136 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain YZ22MPE6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3760696..3771136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIY75_RS18310 aroP 3761694..3763067 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  SIY75_RS18315 ampE 3763110..3763964 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  SIY75_RS18320 ampD 3763961..3764512 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SIY75_RS18325 nadC 3764600..3765493 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SIY75_RS18330 pilA 3765696..3766136 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  SIY75_RS18335 pilB 3766146..3767531 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  SIY75_RS18340 hofC 3767521..3768723 (+) 1203 WP_000157266.1 protein transport protein HofC -
  SIY75_RS18345 guaC 3768758..3769801 (-) 1044 WP_001217338.1 GMP reductase -
  SIY75_RS18350 - 3769957..3770001 (-) 45 WP_120795372.1 protein YacM -
  SIY75_RS18355 coaE 3770026..3770646 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=830190 SIY75_RS18330 WP_000360904.1 3765696..3766136(+) (pilA) [Escherichia coli strain YZ22MPE6]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=830190 SIY75_RS18330 WP_000360904.1 3765696..3766136(+) (pilA) [Escherichia coli strain YZ22MPE6]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363