Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SHK14_RS18740 Genome accession   NZ_CP139337
Coordinates   3795475..3795915 (+) Length   146 a.a.
NCBI ID   WP_029392818.1    Uniprot ID   -
Organism   Escherichia coli strain YZ22MPE54     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3790475..3800915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHK14_RS18720 aroP 3791473..3792846 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  SHK14_RS18725 ampE 3792889..3793743 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  SHK14_RS18730 ampD 3793740..3794291 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SHK14_RS18735 nadC 3794379..3795272 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SHK14_RS18740 pilA 3795475..3795915 (+) 441 WP_029392818.1 prepilin peptidase-dependent pilin Machinery gene
  SHK14_RS18745 pilB 3795925..3797310 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  SHK14_RS18750 hofC 3797300..3798502 (+) 1203 WP_000157266.1 protein transport protein HofC -
  SHK14_RS18755 guaC 3798537..3799580 (-) 1044 WP_001217338.1 GMP reductase -
  SHK14_RS18760 - 3799736..3799780 (-) 45 WP_120795372.1 protein YacM -
  SHK14_RS18765 coaE 3799805..3800425 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15607.73 Da        Isoelectric Point: 4.3938

>NTDB_id=830107 SHK14_RS18740 WP_029392818.1 3795475..3795915(+) (pilA) [Escherichia coli strain YZ22MPE54]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMSPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=830107 SHK14_RS18740 WP_029392818.1 3795475..3795915(+) (pilA) [Escherichia coli strain YZ22MPE54]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GTCACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363