Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SHK14_RS01900 Genome accession   NZ_CP139337
Coordinates   412191..412868 (-) Length   225 a.a.
NCBI ID   WP_000178154.1    Uniprot ID   P25960
Organism   Escherichia coli strain YZ22MPE54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407191..417868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHK14_RS01885 chiA 408558..411251 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  SHK14_RS01890 bfd 411420..411614 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SHK14_RS01895 bfr 411686..412162 (+) 477 WP_000675504.1 bacterioferritin -
  SHK14_RS01900 pilD 412191..412868 (-) 678 WP_000178154.1 prepilin peptidase GspO Machinery gene
  SHK14_RS01905 gspM 412868..413329 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  SHK14_RS01910 gspL 413326..414489 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  SHK14_RS01915 gspK 414504..415487 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  SHK14_RS01920 gspJ 415480..416067 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SHK14_RS01925 gspI 416060..416437 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SHK14_RS01930 gspH 416434..416943 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  SHK14_RS01935 gspG 416951..417388 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24956.82 Da        Isoelectric Point: 7.6353

>NTDB_id=830060 SHK14_RS01900 WP_000178154.1 412191..412868(-) (pilD) [Escherichia coli strain YZ22MPE54]
MTMLLPLFILVGFIADYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=830060 SHK14_RS01900 WP_000178154.1 412191..412868(-) (pilD) [Escherichia coli strain YZ22MPE54]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P25960

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378