Detailed information NTG0830029

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: P4G13_RS21425
• Genome accession: NZ_CP125111
• Coordinates: 4359754..4360554 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 327
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4354754..4365554

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P4G13_RS21390 (P4G13_21345)	gspH	4354907..4355422 (+)	516	WP_021313445.1	type II secretion system minor pseudopilin GspH	-
P4G13_RS21395 (P4G13_21350)	gspI	4355419..4355784 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
P4G13_RS21400 (P4G13_21355)	gspJ	4355781..4356377 (+)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
P4G13_RS21405 (P4G13_21360)	gspK	4356374..4357360 (+)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
P4G13_RS21410 (P4G13_21365)	gspL	4357347..4358543 (+)	1197	WP_064145608.1	type II secretion system protein GspL	-
P4G13_RS21415 (P4G13_21370)	-	4358536..4359021 (+)	486	WP_023312675.1	type II secretion system protein M	-
P4G13_RS21420 (P4G13_21375)	-	4359011..4359757 (+)	747	WP_004145897.1	type II secretion system protein N	-
P4G13_RS21425 (P4G13_21380)	pilD	4359754..4360554 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
P4G13_RS21430 (P4G13_21385)	hrpB	4360602..4363031 (-)	2430	WP_064145607.1	ATP-dependent helicase HrpB	-
P4G13_RS21435 (P4G13_21390)	thpR	4363104..4363640 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
P4G13_RS21440 (P4G13_21395)	sfsA	4363640..4364356 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
P4G13_RS21445 (P4G13_21400)	dksA	4364519..4364974 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=830029 P4G13_RS21425 WP_004145898.1 4359754..4360554(+) (pilD) [Klebsiella pneumoniae strain 327]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=830029 P4G13_RS21425 WP_004145898.1 4359754..4360554(+) (pilD) [Klebsiella pneumoniae strain 327]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425