Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QLF99_RS14020 Genome accession   NZ_CP125110
Coordinates   3033721..3034779 (-) Length   352 a.a.
NCBI ID   WP_054668759.1    Uniprot ID   -
Organism   Stenotrophomonas acidaminiphila strain SPDF1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3028721..3039779
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QLF99_RS13995 (QLF99_13995) - 3028786..3029796 (-) 1011 WP_054667350.1 MoxR family ATPase -
  QLF99_RS14000 (QLF99_14000) - 3029911..3031800 (-) 1890 WP_282883618.1 type IV pilus secretin PilQ family protein -
  QLF99_RS14005 (QLF99_14005) - 3031822..3032313 (-) 492 WP_282884702.1 pilus assembly protein PilP -
  QLF99_RS14010 (QLF99_14010) pilO 3032358..3033035 (-) 678 WP_282883619.1 type 4a pilus biogenesis protein PilO -
  QLF99_RS14015 (QLF99_14015) - 3033032..3033721 (-) 690 WP_282883620.1 PilN domain-containing protein -
  QLF99_RS14020 (QLF99_14020) pilM 3033721..3034779 (-) 1059 WP_054668759.1 pilus assembly protein PilM Machinery gene
  QLF99_RS14025 (QLF99_14025) - 3034997..3037429 (+) 2433 WP_282883621.1 penicillin-binding protein 1A -
  QLF99_RS14030 (QLF99_14030) - 3037508..3038134 (+) 627 WP_282883622.1 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37395.95 Da        Isoelectric Point: 4.4316

>NTDB_id=830011 QLF99_RS14020 WP_054668759.1 3033721..3034779(-) (pilM) [Stenotrophomonas acidaminiphila strain SPDF1]
MGLIPKSQPPIIGVDISSTAVKLLQLARSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAVARSGTRAKGAAAA
VAGSAVITKVIPMPADLDEADLEAQVELEAVNYIPYPIEEVSLDFEVLGPVPNNPEMVQVLLAASRSENVELRQSALELG
GLVAKVMDVEAFAIENAFALVASELPVASDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYEVEVLEPFKEATVQQIGRLLQFFYAGSEFNRVDHIVLAGGCASLPGLPVMVEEQLGVSTSVANPLA
QMTLGQKVQAHALAQDAPALMIATGLALRGFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=830011 QLF99_RS14020 WP_054668759.1 3033721..3034779(-) (pilM) [Stenotrophomonas acidaminiphila strain SPDF1]
GTGGGGCTTATCCCAAAGAGTCAACCACCGATCATCGGTGTTGACATCAGCTCGACTGCGGTAAAGCTGCTTCAGCTTGC
CCGCAGCGGCAATCGTTTTCGCGTGGAACATTACGCCGTGGAACCACTTCCGCCGAATGCGGTGGTGGAGAAGAACATCG
TCGAGGTCGAGGCGGTCGGCGAGGCCATACGCCGGGCCGTGGCGCGCTCGGGAACCCGGGCCAAGGGGGCGGCTGCCGCG
GTCGCCGGTTCGGCGGTGATCACCAAGGTGATCCCGATGCCGGCCGACCTGGACGAGGCCGACCTGGAAGCCCAGGTCGA
GCTGGAGGCGGTGAACTACATCCCGTATCCGATCGAGGAAGTGAGCCTGGACTTCGAGGTGCTCGGGCCGGTGCCCAACA
ACCCGGAAATGGTCCAGGTGCTGTTGGCGGCCTCGCGCTCGGAGAACGTCGAACTGCGCCAGTCGGCGCTGGAGCTGGGC
GGGCTGGTGGCCAAGGTCATGGACGTGGAAGCGTTCGCGATCGAGAACGCCTTCGCGCTGGTGGCCAGCGAACTGCCGGT
GGCCAGTGACGGCGTGGTCGCGCTGGTGGACATCGGCGCCACCATGACCACGCTCAACGTCCTGCGTGGCGGGCGCAGCC
TGTACAGCCGCGAACAGGTGTTCGGCGGCAAGCAGCTCACCGACGAGGTGATGCGCCGCTACGGGCTGAGCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGCGGGCTGCCGGAGAGCTACGAGGTGGAAGTGCTGGAGCCGTTCAAGGAGGCCACGGT
CCAGCAGATCGGCCGCCTGTTGCAGTTCTTCTATGCCGGCAGCGAGTTCAATCGCGTCGATCACATCGTGCTGGCCGGTG
GCTGCGCTTCGCTGCCCGGGCTGCCGGTGATGGTCGAGGAACAACTGGGGGTGAGCACGTCGGTGGCCAATCCGCTGGCG
CAGATGACGCTGGGACAGAAGGTGCAGGCGCATGCGCTGGCCCAGGATGCCCCGGCGCTGATGATCGCCACCGGCCTGGC
CCTGCGGGGGTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50

100

0.5
  comM Acinetobacter nosocomialis M2

50

100

0.5
  comM Acinetobacter baylyi ADP1

50.292

97.159

0.489
  pilM Legionella pneumophila strain ERS1305867

46.571

99.432

0.463