Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SKB19_RS18790 Genome accession   NZ_CP139177
Coordinates   3822987..3823427 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain JX21CE33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3817987..3828427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SKB19_RS18770 (SKB19_18770) aroP 3818985..3820358 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  SKB19_RS18775 (SKB19_18775) ampE 3820401..3821255 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  SKB19_RS18780 (SKB19_18780) ampD 3821252..3821803 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  SKB19_RS18785 (SKB19_18785) nadC 3821891..3822784 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SKB19_RS18790 (SKB19_18790) pilA 3822987..3823427 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  SKB19_RS18795 (SKB19_18795) pilB 3823437..3824822 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  SKB19_RS18800 (SKB19_18800) hofC 3824845..3826014 (+) 1170 WP_225387990.1 protein transport protein HofC -
  SKB19_RS18805 (SKB19_18805) guaC 3826049..3827092 (-) 1044 WP_001217338.1 GMP reductase -
  SKB19_RS18810 (SKB19_18810) - 3827248..3827292 (-) 45 WP_120795372.1 protein YacM -
  SKB19_RS18815 (SKB19_18815) coaE 3827317..3827937 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=829644 SKB19_RS18790 WP_000360904.1 3822987..3823427(+) (pilA) [Escherichia coli strain JX21CE33]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=829644 SKB19_RS18790 WP_000360904.1 3822987..3823427(+) (pilA) [Escherichia coli strain JX21CE33]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363