Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SIY77_RS15995 Genome accession   NZ_CP139125
Coordinates   3283413..3284090 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain YZ22PE244     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3278413..3289090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIY77_RS15980 chiA 3279780..3282473 (+) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -
  SIY77_RS15985 bfd 3282642..3282836 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SIY77_RS15990 bfr 3282908..3283384 (+) 477 WP_000675504.1 bacterioferritin -
  SIY77_RS15995 pilD 3283413..3284090 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  SIY77_RS16000 gspM 3284090..3284551 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  SIY77_RS16005 gspL 3284548..3285711 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  SIY77_RS16010 gspK 3285726..3286709 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  SIY77_RS16015 gspJ 3286702..3287289 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SIY77_RS16020 gspI 3287282..3287659 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SIY77_RS16025 gspH 3287656..3288165 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  SIY77_RS16030 gspG 3288173..3288610 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=829311 SIY77_RS15995 WP_000178161.1 3283413..3284090(-) (pilD) [Escherichia coli strain YZ22PE244]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=829311 SIY77_RS15995 WP_000178161.1 3283413..3284090(-) (pilD) [Escherichia coli strain YZ22PE244]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378