Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   SID94_RS02580 Genome accession   NZ_CP139039
Coordinates   536921..538354 (-) Length   477 a.a.
NCBI ID   WP_001764900.1    Uniprot ID   -
Organism   Escherichia coli MP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 531921..543354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SID94_RS02545 (SID94_02545) elbB 532613..533266 (+) 654 WP_000719791.1 isoprenoid biosynthesis glyoxalase ElbB -
  SID94_RS02550 (SID94_02550) mtgA 533263..533991 (+) 729 WP_032286644.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  SID94_RS02555 (SID94_02555) yrbL 533988..534620 (-) 633 WP_000620407.1 PhoP regulatory network protein YrbL -
  SID94_RS02560 (SID94_02560) npr 534833..535105 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  SID94_RS02565 (SID94_02565) rapZ 535102..535956 (-) 855 WP_000243741.1 RNase adapter RapZ -
  SID94_RS02570 (SID94_02570) ptsN 536002..536493 (-) 492 WP_000183674.1 PTS IIA-like nitrogen regulatory protein PtsN -
  SID94_RS02575 (SID94_02575) hpf 536611..536898 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  SID94_RS02580 (SID94_02580) rpoN/rpoN1 536921..538354 (-) 1434 WP_001764900.1 RNA polymerase factor sigma-54 Machinery gene
  SID94_RS02585 (SID94_02585) lptB 538402..539127 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  SID94_RS02590 (SID94_02590) lptA 539134..539691 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  SID94_RS02595 (SID94_02595) lptC 539660..540235 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  SID94_RS02600 (SID94_02600) kdsC 540232..540798 (-) 567 WP_000030016.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  SID94_RS02605 (SID94_02605) kdsD 540819..541805 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  SID94_RS02610 (SID94_02610) yrbG 541819..542796 (-) 978 WP_000922880.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53992.81 Da        Isoelectric Point: 4.3837

>NTDB_id=829102 SID94_RS02580 WP_001764900.1 536921..538354(-) (rpoN/rpoN1) [Escherichia coli MP1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGNEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=829102 SID94_RS02580 WP_001764900.1 536921..538354(-) (rpoN/rpoN1) [Escherichia coli MP1]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGCAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACACAGACCTTACAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCAATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAGACCACGCCATGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTATTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGATAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGCGAAGAATATATGAAACCGATGGTACTGGCTGATATCGCCCAGGCCG
TCGAGATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACTGAAGGCGGGGGTGAAGCCTCTTCCACAGCGATTCGCGCACTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465