Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SID94_RS01915 Genome accession   NZ_CP139039
Coordinates   417518..418195 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli MP1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412518..423195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SID94_RS01900 (SID94_01900) chiA 413876..416578 (+) 2703 WP_001765222.1 bifunctional chitinase/lysozyme -
  SID94_RS01905 (SID94_01905) bfd 416747..416941 (+) 195 WP_001765223.1 bacterioferritin-associated ferredoxin -
  SID94_RS01910 (SID94_01910) bfr 417013..417489 (+) 477 WP_000675504.1 bacterioferritin -
  SID94_RS01915 (SID94_01915) pilD 417518..418195 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  SID94_RS01920 (SID94_01920) gspM 418195..418656 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  SID94_RS01925 (SID94_01925) gspL 418653..419816 (-) 1164 WP_001765224.1 type II secretion system protein GspL -
  SID94_RS01930 (SID94_01930) gspK 419831..420814 (-) 984 WP_001765225.1 type II secretion system minor pseudopilin GspK -
  SID94_RS01935 (SID94_01935) gspJ 420807..421394 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  SID94_RS01940 (SID94_01940) gspI 421387..421764 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  SID94_RS01945 (SID94_01945) gspH 421761..422270 (-) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  SID94_RS01950 (SID94_01950) gspG 422278..422715 (-) 438 WP_001765226.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=829099 SID94_RS01915 WP_000178175.1 417518..418195(-) (pilD) [Escherichia coli MP1]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=829099 SID94_RS01915 WP_000178175.1 417518..418195(-) (pilD) [Escherichia coli MP1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387