Detailed information NTG0082879

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: H7677_RS00750
• Genome accession: NZ_AP023391
• Coordinates: 117431..117865 (+)
• Length: 144 a.a.
• NCBI ID: WP_079268055.1
• Uniprot ID: -
• Organism: Streptococcus suis strain DAT299
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 112431..122865

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7677_RS00715 (DAT299_01060)	-	113173..113538 (+)	366	WP_002936568.1	DUF1033 family protein	-
H7677_RS00720 (DAT299_01070)	-	113573..114523 (-)	951	WP_184493703.1	S66 peptidase family protein	-
H7677_RS00725 (DAT299_01080)	comYA	114609..115559 (+)	951	WP_184493705.1	competence type IV pilus ATPase ComGA	Machinery gene
H7677_RS00730 (DAT299_01090)	comYB	115471..116508 (+)	1038	WP_269473649.1	competence type IV pilus assembly protein ComGB	Machinery gene
H7677_RS00735 (DAT299_01100)	comYC	116510..116791 (+)	282	WP_184493707.1	competence type IV pilus major pilin ComGC	Machinery gene
H7677_RS00740 (DAT299_01110)	comGD	116772..117179 (+)	408	WP_184493709.1	competence type IV pilus minor pilin ComGD	-
H7677_RS00745 (DAT299_01120)	comYE	117151..117444 (+)	294	WP_011921722.1	competence type IV pilus minor pilin ComGE	Machinery gene
H7677_RS00750 (DAT299_01130)	comGF/cglF	117431..117865 (+)	435	WP_079268055.1	competence type IV pilus minor pilin ComGF	Machinery gene
H7677_RS00755 (DAT299_01140)	comGG	117843..118370 (+)	528	WP_184493711.1	competence type IV pilus minor pilin ComGG	-
H7677_RS00760 (DAT299_01150)	comYH	118427..119380 (+)	954	WP_184493713.1	class I SAM-dependent methyltransferase	Machinery gene
H7677_RS00765 (DAT299_01160)	-	119430..120617 (+)	1188	WP_184493715.1	acetate kinase	-
H7677_RS00770 (DAT299_01170)	-	120932..121483 (+)	552	WP_184493717.1	folate family ECF transporter S component	-
H7677_RS00775 (DAT299_01180)	-	121537..122793 (+)	1257	WP_184493719.1	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16485.85 Da
• Isoelectric Point: 8.8227

>NTDB_id=82879 H7677_RS00750 WP_079268055.1 117431..117865(+) (comGF/cglF) [Streptococcus suis strain DAT299]
MLKSKIPAFTLLECLVALVILSGSLLIFEGLSKLLVQEAHYHRQSVQKEWLVFSSQLRSEWDQSELVKVENGKVYVNKAG
QALAFGKSRSDDFRKTNDRGQGYQPMLYQVDSAAISQENQLVRIDFSFKNGEERTFIYAFKEKS

Nucleotide

• Length: 435 bp

>NTDB_id=82879 H7677_RS00750 WP_079268055.1 117431..117865(+) (comGF/cglF) [Streptococcus suis strain DAT299]
TTGTTAAAAAGTAAAATTCCTGCTTTTACACTCTTGGAATGTTTGGTGGCTCTAGTCATTCTGTCAGGTAGCCTCTTGAT
TTTTGAAGGCTTATCCAAATTACTAGTCCAGGAAGCGCATTATCACCGTCAATCTGTCCAAAAGGAGTGGCTAGTATTTT
CTAGTCAGTTGCGGTCGGAATGGGACCAGTCGGAATTAGTCAAGGTTGAAAATGGCAAGGTCTATGTCAATAAGGCTGGA
CAAGCATTAGCCTTTGGCAAGTCACGGTCGGATGATTTTCGGAAAACCAATGATAGGGGTCAGGGCTACCAACCCATGCT
CTATCAGGTGGATAGCGCAGCCATTTCCCAAGAAAACCAGCTGGTGCGCATCGACTTTAGCTTTAAAAACGGAGAGGAGC
GGACCTTTATCTATGCTTTTAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae TIGR4	54.61	97.917	0.535
comGF/cglF	Streptococcus pneumoniae Rx1	54.61	97.917	0.535
comGF/cglF	Streptococcus pneumoniae D39	54.61	97.917	0.535
comGF/cglF	Streptococcus pneumoniae R6	54.61	97.917	0.535
comGF/cglF	Streptococcus mitis NCTC 12261	55.224	93.056	0.514
comYF	Streptococcus mutans UA140	49.306	100	0.493
comGF/cglF	Streptococcus mitis SK321	52.239	93.056	0.486
comYF	Streptococcus mutans UA159	48.611	100	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.39	97.917	0.444