Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PMI20_RS29325 Genome accession   NZ_CP124732
Coordinates   6534572..6535939 (-) Length   455 a.a.
NCBI ID   WP_007930034.1    Uniprot ID   A0AAX2EB22
Organism   Pseudomonas sp. GM17     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 6529572..6540939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PMI20_RS29300 (PMI20_029300) - 6530416..6530613 (-) 198 WP_007930039.1 YbdD/YjiX family protein -
  PMI20_RS29305 (PMI20_029305) - 6530629..6532695 (-) 2067 WP_007930038.1 carbon starvation CstA family protein -
  PMI20_RS29310 (PMI20_029310) - 6532905..6533366 (+) 462 WP_007930037.1 isoprenylcysteine carboxylmethyltransferase family protein -
  PMI20_RS29315 (PMI20_029315) - 6533420..6533788 (+) 369 WP_007930036.1 PilZ domain-containing protein -
  PMI20_RS29320 (PMI20_029320) - 6533814..6534542 (-) 729 WP_007930035.1 hypothetical protein -
  PMI20_RS29325 (PMI20_029325) radA 6534572..6535939 (-) 1368 WP_007930034.1 DNA repair protein RadA Machinery gene
  PMI20_RS29330 (PMI20_029330) - 6535986..6536534 (-) 549 WP_007930033.1 ankyrin repeat domain-containing protein -
  PMI20_RS29335 (PMI20_029335) katB 6536722..6538263 (-) 1542 WP_007930032.1 catalase KatB -
  PMI20_RS29340 (PMI20_029340) mscL 6538550..6538963 (+) 414 WP_007930031.1 large-conductance mechanosensitive channel protein MscL -
  PMI20_RS29345 (PMI20_029345) - 6539017..6539793 (-) 777 WP_007930029.1 ferredoxin--NADP reductase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48809.29 Da        Isoelectric Point: 7.1317

>NTDB_id=827796 PMI20_RS29325 WP_007930034.1 6534572..6535939(-) (radA) [Pseudomonas sp. GM17]
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLTETMVESGGAAAPSGRTGWAGQQAQIKTLAEVSVEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNIATRMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARQEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEPPPGLRIIAVTRLEQALDALFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=827796 PMI20_RS29325 WP_007930034.1 6534572..6535939(-) (radA) [Pseudomonas sp. GM17]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGCGGCGCAACCTTTCCCAAATGGGCCGGCCAGTGCGGCGAATG
CGGGGCCTGGAACACCCTGACCGAAACCATGGTGGAAAGCGGCGGTGCCGCGGCCCCCAGCGGTCGTACCGGCTGGGCCG
GGCAGCAGGCGCAGATCAAGACCCTGGCCGAGGTCAGCGTCGAAGAGATCCCGCGTTTTTCCACGGCTTCCGGCGAACTG
GACCGGGTGCTCGGCGGCGGCCTGGTCGACGGTTCGGTGGTGCTGATCGGCGGCGACCCGGGGATCGGCAAGTCGACCAT
TTTGTTACAGACTTTGTGCAACATCGCCACGCGCATGCCGGCGCTCTACGTCACCGGCGAGGAATCGCAGCAACAGGTGG
CGATGCGCGCCCGGCGCCTGGGCCTGCCCCAGGATCAGCTGCGGGTGATGACCGAAACCTGTATCGAAACCATCATCGCC
ACGGCCCGGCAGGAGAAGCCCAAGGTCATGGTGATCGACTCGATCCAGACCATCTTCACCGAGCAGCTGCAATCGGCCCC
CGGCGGTGTTTCCCAGGTGCGCGAAAGCGCCGCGCTGCTGGTGCGCTACGCCAAGCAGAGCGGCACGGCGATCTTCCTGG
TGGGCCATGTGACCAAGGAGGGCGCGCTGGCCGGGCCGCGGGTCCTCGAGCACATGGTCGACACGGTGCTGTATTTCGAA
GGTGAATCCGACGGCCGCCTGCGCTTGCTGCGGGCGGTGAAGAACCGTTTCGGCGCGGTCAACGAACTGGGCGTATTTGG
CATGACCGACCGCGGCCTGAAAGAAGTTTCCAATCCTTCGGCGATCTTTCTCACCCGTGCCCAGGAAGAAGTCCCGGGCA
GTGTGGTCATGGCTACCTGGGAAGGCACCCGGCCGATGCTGGTGGAAGTCCAGGCGCTGGTGGATGACAGCCACCTGGCC
AACCCGCGGCGGGTTACCCTGGGCCTGGACCAGAACCGCCTGGCGATGCTGCTGGCGGTACTGCACCGCCACGGCGGCAT
TCCGACCCACGATCAGGACGTGTTCCTCAACGTCGTCGGCGGGGTCAAGGTGCTGGAAACTGCCTCCGACCTGGCGCTGA
TGGCGGCGGTAATGTCGAGCCTGCGCAATCGGCCCTTGCCCCACGACTTGCTGGTGTTCGGCGAAGTCGGCCTGTCTGGT
GAAGTGCGCCCGGTGCCGAGCGGCCAGGAGCGCCTCAAGGAAGCGGCCAAGCACGGCTTCAAACGGGCCATCGTGCCCAA
GGGCAATGCCCCTAAAGAGCCACCGCCGGGCTTGCGAATCATTGCGGTAACCCGTCTGGAGCAGGCGCTGGATGCGCTGT
TCGAATGA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

AAA domain (AAA_25)

(80-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.796

100

0.49
  radA Streptococcus pneumoniae Rx1

45.87

100

0.464
  radA Streptococcus pneumoniae R6

45.87

100

0.464
  radA Streptococcus pneumoniae TIGR4

45.87

100

0.464
  radA Streptococcus pneumoniae D39

45.87

100

0.464
  radA Streptococcus mitis SK321

46.053

100

0.462
  radA Streptococcus mitis NCTC 12261

45.833

100

0.459