Detailed information NTG0826935

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P7I84_RS02030
• Genome accession: NZ_CP124638
• Coordinates: 437333..438481 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain 2021CK-01158
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 432333..443481

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7I84_RS02005 (P7I84_02005)	-	433484..433780 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
P7I84_RS02010 (P7I84_02010)	-	433777..434370 (-)	594	WP_058150765.1	YggT family protein	-
P7I84_RS02015 (P7I84_02015)	proC	434381..435202 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
P7I84_RS02020 (P7I84_02020)	-	435214..435906 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
P7I84_RS02025 (P7I84_02025)	pilT	436121..437155 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
P7I84_RS02030 (P7I84_02030)	pilU	437333..438481 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
P7I84_RS02035 (P7I84_02035)	czcD	438489..439388 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
P7I84_RS02040 (P7I84_02040)	-	439542..439946 (+)	405	WP_003084560.1	NINE protein	-
P7I84_RS02045 (P7I84_02045)	-	440189..441562 (+)	1374	WP_023098360.1	pyridoxal-phosphate dependent enzyme	-
P7I84_RS02050 (P7I84_02050)	-	441559..442743 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=826935 P7I84_RS02030 WP_003100959.1 437333..438481(+) (pilU) [Pseudomonas aeruginosa strain 2021CK-01158]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=826935 P7I84_RS02030 WP_003100959.1 437333..438481(+) (pilU) [Pseudomonas aeruginosa strain 2021CK-01158]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGAGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTACACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGATCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382