NTDB

Overview

Name	radA	Type	Machinery gene
Locus tag	QA539_RS10145	Genome accession	NZ_CP124591
Coordinates	1933609..1934970 (-)	Length	453 a.a.
NCBI ID	WP_219493319.1	Uniprot ID	-
Organism	Macrococcus sp. CCM 8659
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1928609..1939970

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QA539_RS10120 (QA539_10100)	cysE	1929035..1929688 (-)	654	WP_219493313.1	serine O-acetyltransferase	-
QA539_RS10125 (QA539_10105)	gltX	1929926..1931380 (-)	1455	WP_419895177.1	glutamate--tRNA ligase	-
QA539_RS10130 (QA539_10110)	ispF	1931398..1931877 (-)	480	WP_147404566.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
QA539_RS10135 (QA539_10115)	ispD	1931874..1932554 (-)	681	WP_419895178.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-
QA539_RS10140 (QA539_10120)	-	1932547..1933596 (-)	1050	WP_219493317.1	PIN/TRAM domain-containing protein	-
QA539_RS10145 (QA539_10125)	radA	1933609..1934970 (-)	1362	WP_219493319.1	DNA repair protein RadA	Machinery gene
QA539_RS10150 (QA539_10130)	-	1935194..1937620 (-)	2427	WP_219494863.1	ATP-dependent Clp protease ATP-binding subunit	-
QA539_RS10155 (QA539_10135)	-	1937630..1938634 (-)	1005	WP_118003427.1	protein arginine kinase	-
QA539_RS10160 (QA539_10140)	-	1938624..1939010 (-)	387	WP_219493322.1	UvrB/UvrC motif-containing protein	-
QA539_RS10165 (QA539_10145)	-	1939021..1939482 (-)	462	WP_219493324.1	CtsR family transcriptional regulator	-

Sequence

Protein

Download Length: 453 a.a. Molecular weight: 50224.90 Da Isoelectric Point: 7.4631

                                >NTDB_id=826539 QA539_RS10145 WP_219493319.1 1933609..1934970(-) (radA) [Macrococcus sp. CCM 8659]

MVKVKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKNAISESQSGNKVEKLKDITKESVPRDHTQMKELDR

VLGGGIVPGSLILIGGDPGIGKSTLLLQVCAMLSQNHPVLYISGEESVRQTKLRADRLLEDAGELDVYAETNLQIIHETV

RKTKPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD

THHSYRILRAVKNRFGSTNEMGIFEMKNTGLKEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVTPTSFHNP

RRMASGVDHNRLNLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSVIVSIASSYNDKPTRGDDCFIGEVGLTGEV

RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNINEALKMAF

Nucleotide

Download Length: 1362 bp

                                >NTDB_id=826539 QA539_RS10145 WP_219493319.1 1933609..1934970(-) (radA) [Macrococcus sp. CCM 8659]

TTGGTAAAGGTGAAAAGCACATTTGAATGTATGGCCTGTGGTTATCAGTCACCGAAATGGATGGGAAAGTGTCCGAATTG

TGGTGCATGGAATCAAATGGAAGAAGTTATTGAGCATAAACAGAAGGGCCCTAAAAATGCGATTTCTGAATCACAAAGTG

GTAATAAAGTTGAAAAACTAAAAGATATAACGAAAGAAAGTGTTCCTCGTGATCATACGCAGATGAAAGAACTGGATCGT

GTACTTGGAGGAGGAATCGTTCCGGGTTCATTGATACTTATCGGCGGAGACCCGGGGATTGGTAAGTCCACATTACTACT

ACAAGTTTGTGCAATGCTTTCACAAAACCATCCCGTACTCTATATTTCTGGAGAGGAATCGGTAAGACAAACGAAATTAC

GTGCCGATCGTCTATTAGAGGATGCAGGTGAGCTTGATGTATATGCAGAAACCAACCTTCAGATTATCCACGAAACGGTA

AGGAAAACAAAACCGAAATTTTTGGTTATCGATTCAATTCAAACAATATTTCATCCAGAGGTTACAAGCGCACCTGGTTC

TGTATCGCAAGTCAGAGAATGTACACAAGAGTTAATGCGTATCGCAAAGCAGATGAACATTGCAACATTTATCGTAGGGC

ACGTGACAAAAGAAGGACAGATCGCAGGTCCAAGGTTACTAGAACATATGGTTGATACAGTTCTTTACTTTGAAGGAGAT

ACTCATCATAGCTACAGAATATTACGCGCAGTGAAGAATCGTTTTGGTTCGACAAATGAAATGGGTATATTTGAAATGAA

GAATACAGGCCTTAAAGAAGTCCTGAATCCTTCAGAAATGTTCCTTGAAGAACGTACAAAAAATGTAGCAGGTTCAACTA

TCGTTGCAACGATGGAAGGCACGCGTCCATTACTAGTTGAAGTTCAGTCACTTGTAACGCCGACATCTTTTCATAATCCA

AGAAGAATGGCATCAGGTGTCGACCATAATCGACTGAACCTATTGATGGCTGTACTAGAGAAAAAACAAGGATATTTATT

ACAGCAACAGGATGCATATGTGAAAGTTGCGGGTGGTGTAAAACTTGATGAACCTGCTGTTGATTTAAGTGTTATCGTAA

GCATCGCTTCAAGCTATAATGATAAACCGACACGAGGCGATGACTGTTTTATCGGAGAAGTCGGTCTGACTGGTGAAGTG

AGACGTGTTGCACGTATTGAACAACGTGTACAAGAAGCTGAAAAACTCGGTTTTAAACGTGTCATCATCCCAAAAAATAA

TATTGGTGGCTGGGATTTCCCCGGAAATATTGAAGTGATTGGTGTAACAAATATTAACGAAGCATTAAAAATGGCATTTT

AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
radA	Bacillus subtilis subsp. subtilis str. 168	66.228	100	0.667
radA	Streptococcus pneumoniae Rx1	62.555	100	0.627
radA	Streptococcus pneumoniae D39	62.555	100	0.627
radA	Streptococcus pneumoniae R6	62.555	100	0.627
radA	Streptococcus pneumoniae TIGR4	62.555	100	0.627
radA	Streptococcus mitis NCTC 12261	62.555	100	0.627
radA	Streptococcus mitis SK321	62.555	100	0.627

Detailed information NTG0826539