Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SE698_RS19375 Genome accession   NZ_CP138428
Coordinates   4019314..4019991 (+) Length   225 a.a.
NCBI ID   WP_000178176.1    Uniprot ID   -
Organism   Escherichia coli strain 6EVA     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4014314..4024991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE698_RS19340 gspG 4014794..4015231 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  SE698_RS19345 gspH 4015239..4015748 (+) 510 WP_001076045.1 type II secretion system minor pseudopilin GspH -
  SE698_RS19350 gspI 4015745..4016122 (+) 378 WP_001041740.1 type II secretion system minor pseudopilin GspI -
  SE698_RS19355 gspJ 4016115..4016702 (+) 588 WP_000610631.1 type II secretion system minor pseudopilin GspJ -
  SE698_RS19360 gspK 4016695..4017678 (+) 984 WP_001058527.1 type II secretion system minor pseudopilin GspK -
  SE698_RS19365 gspL 4017693..4018856 (+) 1164 WP_001115231.1 type II secretion system protein GspL -
  SE698_RS19370 gspM 4018853..4019314 (+) 462 WP_001491350.1 type II secretion system protein GspM -
  SE698_RS19375 pilD 4019314..4019991 (+) 678 WP_000178176.1 prepilin peptidase GspO Machinery gene
  SE698_RS19380 bfr 4020020..4020496 (-) 477 WP_000675504.1 bacterioferritin -
  SE698_RS19385 bfd 4020568..4020762 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SE698_RS19390 chiA 4020931..4023624 (-) 2694 WP_319054158.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24838.69 Da        Isoelectric Point: 7.9585

>NTDB_id=826485 SE698_RS19375 WP_000178176.1 4019314..4019991(+) (pilD) [Escherichia coli strain 6EVA]
MTMLLPLFILVGFIAGYFVNVVAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALTPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=826485 SE698_RS19375 WP_000178176.1 4019314..4019991(+) (pilD) [Escherichia coli strain 6EVA]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCTGGTTATTTTGTTAATGTCGTGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACAGCGTTAACGTTTCGCCAGGTATTAGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATTGCCTGCGCCCTGACACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTATACGGCGCGGTAGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAAGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATTGCATTCGGCCCATGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

35.458

100

0.396
  pilD Neisseria gonorrhoeae MS11

33.721

100

0.387