Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SE700_RS07850 Genome accession   NZ_CP138427
Coordinates   1486342..1487019 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 21EVA     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1481342..1492019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE700_RS07835 chiA 1482709..1485402 (+) 2694 WP_000773141.1 bifunctional chitinase/lysozyme -
  SE700_RS07840 bfd 1485571..1485765 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SE700_RS07845 bfr 1485837..1486313 (+) 477 WP_000675504.1 bacterioferritin -
  SE700_RS07850 pilD 1486342..1487019 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  SE700_RS07855 gspM 1487019..1487480 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  SE700_RS07860 gspL 1487477..1488640 (-) 1164 WP_001115235.1 type II secretion system protein GspL -
  SE700_RS07865 gspK 1488655..1489638 (-) 984 WP_319791433.1 type II secretion system minor pseudopilin GspK -
  SE700_RS07870 gspJ 1489631..1490218 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SE700_RS07875 gspI 1490211..1490588 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SE700_RS07880 gspH 1490585..1491094 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  SE700_RS07885 gspG 1491102..1491539 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=826378 SE700_RS07850 WP_000178161.1 1486342..1487019(-) (pilD) [Escherichia coli strain 21EVA]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=826378 SE700_RS07850 WP_000178161.1 1486342..1487019(-) (pilD) [Escherichia coli strain 21EVA]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378