Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SE692_RS20845 Genome accession   NZ_CP138423
Coordinates   4137791..4138468 (+) Length   225 a.a.
NCBI ID   WP_025670758.1    Uniprot ID   -
Organism   Escherichia coli strain 30EVA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4132791..4143468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE692_RS20810 gspG 4133271..4133708 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  SE692_RS20815 gspH 4133716..4134225 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  SE692_RS20820 gspI 4134222..4134599 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SE692_RS20825 gspJ 4134592..4135179 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SE692_RS20830 gspK 4135172..4136155 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  SE692_RS20835 gspL 4136170..4137333 (+) 1164 WP_032202348.1 type II secretion system protein GspL -
  SE692_RS20840 gspM 4137330..4137791 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  SE692_RS20845 pilD 4137791..4138468 (+) 678 WP_025670758.1 prepilin peptidase GspO Machinery gene
  SE692_RS20850 bfr 4138497..4138973 (-) 477 WP_000675504.1 bacterioferritin -
  SE692_RS20855 bfd 4139045..4139239 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SE692_RS20860 chiA 4139408..4142101 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24888.75 Da        Isoelectric Point: 7.9563

>NTDB_id=826317 SE692_RS20845 WP_025670758.1 4137791..4138468(+) (pilD) [Escherichia coli strain 30EVA1]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLSDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=826317 SE692_RS20845 WP_025670758.1 4137791..4138468(+) (pilD) [Escherichia coli strain 30EVA1]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGTCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378