Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SE702_RS14085 Genome accession   NZ_CP138407
Coordinates   2846507..2847184 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 50EVAW     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2841507..2852184
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE702_RS14050 gspG 2841987..2842424 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  SE702_RS14055 gspH 2842432..2842941 (+) 510 WP_114505551.1 type II secretion system minor pseudopilin GspH -
  SE702_RS14060 gspI 2842938..2843315 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SE702_RS14065 gspJ 2843308..2843895 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SE702_RS14070 gspK 2843888..2844871 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  SE702_RS14075 gspL 2844886..2846049 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  SE702_RS14080 gspM 2846046..2846507 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  SE702_RS14085 pilD 2846507..2847184 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  SE702_RS14090 bfr 2847213..2847689 (-) 477 WP_000675504.1 bacterioferritin -
  SE702_RS14095 bfd 2847761..2847955 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SE702_RS14100 chiA 2848124..2850496 (-) 2373 Protein_2736 bifunctional chitinase/lysozyme -
  SE702_RS14105 tuf 2850558..2851742 (-) 1185 WP_000031783.1 elongation factor Tu -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=825975 SE702_RS14085 WP_000178161.1 2846507..2847184(+) (pilD) [Escherichia coli strain 50EVAW]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=825975 SE702_RS14085 WP_000178161.1 2846507..2847184(+) (pilD) [Escherichia coli strain 50EVAW]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378