Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   SE699_RS03380 Genome accession   NZ_CP138399
Coordinates   721748..722425 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain 64EVA     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 716748..727425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SE699_RS03365 chiA 718115..720808 (+) 2694 WP_319755160.1 bifunctional chitinase/lysozyme -
  SE699_RS03370 bfd 720977..721171 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  SE699_RS03375 bfr 721243..721719 (+) 477 WP_000675504.1 bacterioferritin -
  SE699_RS03380 pilD 721748..722425 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  SE699_RS03385 gspM 722425..722886 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  SE699_RS03390 gspL 722883..724046 (-) 1164 WP_001618895.1 type II secretion system protein GspL -
  SE699_RS03395 gspK 724061..725044 (-) 984 WP_319755161.1 type II secretion system minor pseudopilin GspK -
  SE699_RS03400 gspJ 725037..725624 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  SE699_RS03405 gspI 725617..725994 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  SE699_RS03410 gspH 725991..726500 (-) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  SE699_RS03415 gspG 726508..726945 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=825876 SE699_RS03380 WP_000178161.1 721748..722425(-) (pilD) [Escherichia coli strain 64EVA]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=825876 SE699_RS03380 WP_000178161.1 721748..722425(-) (pilD) [Escherichia coli strain 64EVA]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378