Detailed information NTG0825009

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: SE116_RS04425
• Genome accession: NZ_CP138362
• Coordinates: 923459..927112 (+)
• Length: 1217 a.a.
• NCBI ID: WP_319162521.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain V315
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 918459..932112

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SE116_RS04410 (SE116_04410)	lepB	918707..919231 (+)	525	WP_000758212.1	signal peptidase I	-
SE116_RS04415 (SE116_04415)	lepB	919247..919822 (+)	576	WP_000711897.1	signal peptidase I	-
SE116_RS04420 (SE116_04420)	addB	919982..923458 (+)	3477	WP_000172364.1	helicase-exonuclease AddAB subunit AddB	-
SE116_RS04425 (SE116_04425)	addA	923459..927112 (+)	3654	WP_319162521.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
SE116_RS04430 (SE116_04430)	-	927278..928180 (+)	903	WP_000670752.1	fumarylacetoacetate hydrolase family protein	-
SE116_RS04435 (SE116_04435)	-	928507..928896 (+)	390	WP_000902814.1	YisL family protein	-
SE116_RS04440 (SE116_04440)	-	929067..930383 (-)	1317	WP_001124499.1	CoA-disulfide reductase	-
SE116_RS04445 (SE116_04445)	-	930435..931259 (-)	825	WP_001182436.1	Cof-type HAD-IIB family hydrolase	-
SE116_RS04450 (SE116_04450)	-	931373..931681 (+)	309	WP_000390575.1	metal-sulfur cluster assembly factor	-

Sequence

Protein

• Length: 1217 a.a.
• Molecular weight: 141175.68 Da
• Isoelectric Point: 4.9466

>NTDB_id=825009 SE116_RS04425 WP_319162521.1 923459..927112(+) (addA) [Staphylococcus aureus strain V315]
MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVERIIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRI
QEASIADPANAHLKNQRIKIHQAQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEAIEQHYDILDPAF
IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLTDLSKVFITAAYDALNKAYDLFS
MMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGHEFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSD
YFSREADDLKADMQQLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYRQHFQEILV
DEYQDTNRVQEKILSCIKTGDEHNGNLFMVGDVKQSIYKFRQADPSLFIEKYQRFTIDGDGTGRRIDLSQNFRSRKEVLS
TTNYIFKHMMDEQVGEVNYDESAQLYYGAPYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMK
TGSYRSATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPLQDIYLVGLMRSVIYQF
KEDELAQIRILSPNDDYFYQSIVNYINDEAADAILVDKLKMFLSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIG
GRGRRANLYGLFNKAIEFENSSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLSKD
FNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYRAVAEKELVSEEMRLVYVALTRAKEQLYLIGRVKNDKSLLE
LEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQSASIPDDLKFEKDIAQIEDSSRPNVNISIVYFEDVSTETILDNDEY
RSVNQLETMQNGNEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFSTYERPKFLSEQ
GKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADAKKDIRMDEIMTFINSKLYSIIAEAEQVYRELP
FVVNQALVDQLPQGDEDVSIIQGMIDLIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILN
KEVKGYLYFFKFGTLQL

Nucleotide

• Length: 3654 bp

>NTDB_id=825009 SE116_RS04425 WP_319162521.1 923459..927112(+) (addA) [Staphylococcus aureus strain V315]
ATGACAATTCCAGAGAAACCACAAGGCGTGATTTGGACTGACGCGCAATGGCAAAGTATTTACGCAACTGGACAAGATGT
ACTTGTTGCAGCCGCGGCAGGTTCAGGTAAAACAGCTGTACTAGTTGAGCGTATTATCCAAAAGATTTTACGTGATGGCA
TTGATGTCGATCGACTTTTAGTCGTAACGTTCACAAACTTAAGTGCACGTGAAATGAAGCATCGTGTAGACCAACGTATT
CAAGAGGCATCGATTGCTGATCCTGCAAATGCACACTTGAAAAACCAACGCATCAAAATTCATCAAGCACAAATATCTAC
ACTTCATAGTTTTTGCTTGAAATTAATTCAACAGCATTATGATGTATTAAATATTGACCCGAACTTTAGAACAAGCAGTG
AAGCTGAAAATATTTTATTATTAGAACAAACGATAGATGAAGCCATAGAACAACATTACGATATCCTTGATCCTGCTTTT
ATTGAATTAACAGAGCAATTGTCTTCAGATAGAAGTGATGATCAGTTTCGAATGATTATTAAACAATTGTATTTCTTTAG
CGTTGCAAATCCAAATCCTACAAATTGGTTGGATCAATTGGTGACACCATACGAAGAAGAAGCACAACAAGCGCAACTTA
TTCAACTACTAACAGACTTATCTAAAGTATTTATCACAGCTGCATATGATGCTTTAAATAAGGCGTATGATTTGTTTAGT
ATGATGGATGGCGTCGATAAACATTTAGCTGTTATAGAAGATGAACGACGTTTAATGGGGCGTGTTTTAGAAGGTGGCTT
TATTGATATACCTTATTTAACTGGTCACGAATTTGGCGCGCGTTTGCCTAATGTAACAGCGAAAATTAAAGAAGCAAATG
AAATGATGGTCGATGCCTTAGAAGATGCTAAACTTCAGTATAAAAAATATAAATCATTAATTGATAAAGTGAAAAGTGAT
TACTTTTCAAGAGAAGCTGATGATTTGAAAGCTGATATGCAACAATTGGCGCCACGAGTAAAGTACCTTGCGCGTATTGT
GAAAGATGTTATGTCAGAATTCAATCGAAAAAAGCGTAGCAAAAATATTTTGGATTTTTCTGATTATGAACATTTTGCAT
TACAAATTTTAACTAATGAGGATGGTTCGCCTTCAGAAATTGCCGAATCATACCGTCAACACTTCCAAGAAATATTGGTC
GATGAGTATCAAGATACGAACCGAGTTCAAGAGAAAATATTATCTTGTATCAAAACGGGTGATGAACATAATGGTAATTT
ATTTATGGTTGGAGATGTTAAGCAATCTATTTATAAATTTAGACAAGCTGATCCAAGTTTATTTATTGAAAAGTATCAAC
GCTTTACTATAGATGGAGATGGCACTGGACGTCGAATTGATTTGTCGCAAAACTTCCGTTCACGTAAAGAAGTACTGTCA
ACGACAAACTATATATTCAAACATATGATGGATGAACAAGTCGGTGAAGTAAATTATGATGAATCAGCACAATTGTATTA
TGGTGCACCATATGATGAATCGGACCATCCAGTAAACTTAAAAGTGCTTGTTGAAGCGGATCAAGAACATAGTGATTTAA
CTGGTAGTGAACAAGAAGCGCATTTTATAGTAGAACAAGTTAAAGATATCTTAGAACATCAAAAAGTTTATGATATGAAA
ACAGGAAGCTATAGAAGTGCGACATACAAGGATATCGTTATTCTAGAACGCAGCTTTGGGCAAGCTCGCAATTTACAACA
AGCCTTTAAAAATGAAGATATTCCGTTCCATGTGAATAGTCGTGAAGGTTACTTTGAACAAACAGAAGTCCGTTTAGTAT
TATCATTTTTAAGAGCGATAGATAATCCATTACAAGATATTTATTTAGTTGGGTTAATGCGCTCCGTTATATATCAGTTC
AAAGAAGACGAATTAGCTCAAATTAGAATATTGAGTCCAAATGATGACTACTTCTATCAATCGATTGTAAATTACATTAA
TGACGAAGCAGCAGATGCCATTTTAGTTGATAAATTAAAAATGTTTTTATCAGATATTCAAAGTTACCAACAATATAGTA
AAGATCATCCGGTGTATCAGTTAATTGATAAATTTTATAATGATCATTATGTTATTCAATACTTTAGTGGACTTATTGGT
GGACGTGGACGACGTGCAAATCTTTATGGTTTATTTAATAAAGCTATCGAGTTTGAGAATTCAAGTTTTAGAGGTTTATA
TCAATTTATTCGTTTTATCGATGAATTGATTGAAAGAGGCAAAGATTTTGGTGAGGAAAACGTAGTTGGTCCAAACGATA
ATGTCGTTAGAATGATGACAATTCATAGTAGTAAAGGTCTAGAGTTTCCGTTTGTCATTTATTCTGGATTGTCAAAAGAT
TTTAATAAACGTGATTTGAAACAACCAGTTATTTTAAATCAGCAATTTGGTCTCGGAATGGATTATTTTGATGTGGATAA
AGAAATGGCATTTCCATCTTTAGCTTCGGTTGCATATAGAGCTGTTGCTGAAAAAGAACTTGTGTCAGAAGAAATGCGAT
TAGTCTATGTAGCATTAACAAGAGCGAAAGAACAACTTTATTTAATTGGTAGAGTGAAAAATGATAAATCATTACTAGAA
CTAGAGCAATTGTCTATTTCTGGTGAGCACATTGCTGTCAATGAACGATTAACTTCACCAAATCCGTTCCATCTTATTTA
TAGTATTTTATCTAAACATCAATCTGCGTCAATTCCAGATGATTTAAAATTTGAAAAAGATATAGCACAAATTGAAGATA
GTAGTCGTCCGAATGTAAATATTTCAATTGTGTACTTTGAAGATGTGTCTACAGAAACCATTTTAGATAATGATGAATAT
CGTTCGGTTAATCAATTAGAAACTATGCAAAATGGTAATGAAGATGTTAAAGCACAAATTAAACACCAACTTGATTATCA
ATATCCATATGTAAATGATACTAAAAAGCCCTCAAAACAATCTGTTTCTGAATTGAAAAGACAATATGAAACAGAAGAAA
GTGGCACAAGTTACGAACGAGTAAGGCAATATCGTATCGGTTTTTCAACGTATGAACGACCTAAATTTCTAAGTGAACAA
GGTAAACGAAAAGCGAATGAAATTGGTACGTTAATGCATACAGTGATGCAACATTTACCATTCAAAAAAGAACGCATATC
TGAAGTTGAGTTACATCAGTATATCGATGGATTAATCGATAAACATATTATCGAAGCAGATGCGAAAAAAGATATCCGTA
TGGATGAAATAATGACATTTATCAATAGTAAGTTATATTCGATTATTGCTGAAGCAGAGCAAGTTTATCGTGAATTACCG
TTTGTAGTTAACCAAGCATTAGTGGATCAATTGCCACAAGGAGACGAAGACGTCTCAATTATTCAAGGTATGATTGACTT
GATCTTTGTTAAAGACGGTGTGCATTATTTTGTAGACTATAAAACCGATGCATTTAATCGTCGCCGTGGGATGACAGATG
AAGAAATTGGTACACAATTAAAAAATAAATATAAGATACAGATGAAATATTATCAAAATACGCTTCAAACGATTCTTAAT
AAAGAAGTTAAAGGTTATTTATACTTCTTCAAATTTGGTACATTGCAACTGTAG

Domains

Predicted by InterProScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (466-869)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (12-455)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1040-1210)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	36.725	100	0.38