Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R3F77_RS00775 Genome accession   NZ_CP138302
Coordinates   176565..178178 (+) Length   537 a.a.
NCBI ID   WP_028155459.1    Uniprot ID   -
Organism   Bradyrhizobium japonicum strain CNPSo 31     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 171565..183178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3F77_RS00755 (R3F77_00755) - 173003..173617 (+) 615 WP_014490894.1 ribonuclease D -
  R3F77_RS00760 (R3F77_00760) lptC 173764..174495 (+) 732 WP_038932733.1 LPS export ABC transporter periplasmic protein LptC -
  R3F77_RS00765 (R3F77_00765) - 174501..175217 (+) 717 WP_039158188.1 LptA/OstA family protein -
  R3F77_RS00770 (R3F77_00770) lptB 175464..176474 (+) 1011 WP_038932734.1 LPS export ABC transporter ATP-binding protein -
  R3F77_RS00775 (R3F77_00775) rpoN/rpoN1 176565..178178 (+) 1614 WP_028155459.1 RNA polymerase factor sigma-54 Machinery gene
  R3F77_RS00780 (R3F77_00780) hpf 178263..178862 (+) 600 WP_014490899.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R3F77_RS00785 (R3F77_00785) ptsN 179084..179545 (+) 462 WP_008141251.1 PTS IIA-like nitrogen regulatory protein PtsN -
  R3F77_RS00790 (R3F77_00790) - 179821..181557 (+) 1737 WP_039155184.1 ShlB/FhaC/HecB family hemolysin secretion/activation protein -

Sequence

Protein


Download         Length: 537 a.a.        Molecular weight: 58888.94 Da        Isoelectric Point: 4.5192

>NTDB_id=824428 R3F77_RS00775 WP_028155459.1 176565..178178(+) (rpoN/rpoN1) [Bradyrhizobium japonicum strain CNPSo 31]
MALTQRLEFRQSQSLVMTPQLMQAIKLLQLSNLDLTTFVEEELERNPLLERANDEAPAGEAPAETGQFSDSDGGHNDEPG
GGPGEAFEPGQEEWMSKDLGTRAEIEQTLDTGLDNVFSEEPAEAAARNAQDAAPTTYTEWGGGASGDEDYNLEAFVAAEI
TLGDHLAEQLSVAFTGPAQRMIGQYLIDLVDEAGYLPPDLGQAAERLGASQADVEDVLAVLQKFDPPGVCARNLSECLAI
QLRELDRYDPAMQALVEHLDLLAKRDIASLRKLCGVDDEDIADMIGEIRRLNPKPGMKFGSARLQTMVPDVYVRPGPDGG
WHVELNSDTLPRVLVNQTYYSELSKKIGKDGDKSYFTDALQNATWLVRALDQRARTILKVATEIVRQQDGFFTHGVAHLR
PLNLKAVADAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIASADGGEAHSAEAVRHHIKQLIDSEAPAAILSDDTI
VERLRASGIDIARRTVAKYREAMRIPSSVQRRRDKQSALGNVLSTAMPDRSRNTEPA

Nucleotide


Download         Length: 1614 bp        

>NTDB_id=824428 R3F77_RS00775 WP_028155459.1 176565..178178(+) (rpoN/rpoN1) [Bradyrhizobium japonicum strain CNPSo 31]
ATGGCGCTCACGCAGAGATTAGAGTTCCGGCAATCGCAGTCGCTGGTCATGACGCCGCAGCTGATGCAGGCGATCAAGCT
GCTGCAATTGTCCAATCTCGATCTCACGACCTTCGTGGAAGAGGAACTCGAGCGGAACCCCCTGCTGGAGCGGGCCAACG
ACGAGGCCCCCGCGGGTGAAGCCCCGGCCGAGACCGGCCAGTTCAGCGATTCCGACGGCGGCCACAACGACGAGCCGGGC
GGCGGTCCGGGCGAGGCGTTCGAGCCGGGCCAGGAAGAATGGATGAGCAAGGATCTCGGCACCCGCGCCGAGATCGAGCA
GACCCTGGATACGGGCCTGGACAACGTCTTCTCCGAGGAACCGGCCGAGGCCGCCGCGCGCAACGCCCAGGACGCGGCGC
CGACCACCTATACCGAATGGGGCGGCGGTGCCTCCGGCGACGAGGATTACAATCTCGAAGCCTTCGTCGCCGCGGAGATC
ACGCTCGGCGATCATCTTGCCGAGCAGCTGTCGGTCGCCTTCACCGGCCCGGCGCAGCGCATGATCGGCCAGTACCTGAT
CGACCTCGTCGACGAGGCCGGCTATCTGCCGCCGGATCTCGGCCAGGCCGCCGAGCGCCTTGGCGCGTCGCAAGCCGACG
TCGAGGACGTCCTTGCCGTGCTGCAAAAATTCGATCCGCCCGGCGTCTGCGCGCGCAATTTGAGCGAATGCCTGGCGATC
CAGCTCCGCGAGCTCGACCGCTACGACCCGGCGATGCAGGCCCTCGTCGAGCACCTCGATCTGCTGGCGAAGCGCGACAT
CGCTTCGTTGCGCAAGCTCTGCGGCGTCGACGACGAGGACATCGCCGACATGATCGGCGAGATCCGCCGCCTCAATCCCA
AGCCCGGCATGAAGTTCGGCTCGGCGCGGCTCCAGACCATGGTGCCCGACGTCTATGTCCGCCCGGGCCCGGATGGCGGC
TGGCATGTCGAGCTCAACAGCGACACCTTGCCGCGCGTGCTGGTCAACCAGACCTATTATTCCGAGCTGTCGAAGAAGAT
CGGCAAGGACGGCGACAAGTCCTATTTCACCGACGCGCTCCAGAACGCGACCTGGCTGGTGCGCGCGCTCGATCAGCGCG
CCCGCACCATCCTGAAAGTTGCAACGGAAATCGTGCGCCAGCAGGACGGCTTCTTCACCCACGGCGTGGCGCATCTGCGG
CCGCTGAATCTGAAGGCCGTGGCCGACGCCATCCAGATGCATGAATCGACGGTGTCGCGCGTCACCGCCAACAAGTACAT
GGCGACAAATCGCGGCACATTCGAATTGAAATATTTCTTCACGGCCTCGATCGCCTCCGCCGACGGCGGCGAGGCGCATT
CCGCCGAGGCCGTGCGCCACCACATCAAGCAGCTGATCGATTCGGAAGCGCCGGCGGCGATCCTGTCGGATGACACCATC
GTGGAACGCTTACGCGCCTCGGGAATTGATATTGCCCGCCGCACGGTCGCGAAGTATCGCGAAGCCATGCGCATTCCCTC
GTCGGTACAACGCCGCCGTGACAAGCAGAGCGCTCTTGGTAACGTCCTCTCCACCGCAATGCCCGATCGCTCCCGCAACA
CCGAGCCGGCCTGA

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(354-513)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(6-50)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(152-339)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

36.969

100

0.372