Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R7234_RS21165 Genome accession   NZ_CP137737
Coordinates   4141346..4141786 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain HNSQ2209     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4136346..4146786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R7234_RS21145 (R7234_21145) aroP 4137344..4138717 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  R7234_RS21150 (R7234_21150) ampE 4138760..4139614 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  R7234_RS21155 (R7234_21155) ampD 4139611..4140162 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R7234_RS21160 (R7234_21160) nadC 4140250..4141143 (+) 894 WP_063100732.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R7234_RS21165 (R7234_21165) pilA 4141346..4141786 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  R7234_RS21170 (R7234_21170) pilB 4141796..4143181 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  R7234_RS21175 (R7234_21175) hofC 4143171..4144373 (+) 1203 WP_000157266.1 protein transport protein HofC -
  R7234_RS21180 (R7234_21180) guaC 4144408..4145451 (-) 1044 WP_001217338.1 GMP reductase -
  R7234_RS21185 (R7234_21185) - 4145607..4145651 (-) 45 WP_120795372.1 protein YacM -
  R7234_RS21190 (R7234_21190) coaE 4145676..4146296 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=823974 R7234_RS21165 WP_000360904.1 4141346..4141786(+) (pilA) [Escherichia coli strain HNSQ2209]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=823974 R7234_RS21165 WP_000360904.1 4141346..4141786(+) (pilA) [Escherichia coli strain HNSQ2209]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363