Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   R6I08_RS01925 Genome accession   NZ_CP137696
Coordinates   413258..413935 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli isolate FELIX_MS533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408258..418935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R6I08_RS01910 (R6I08_01910) chiA 409625..412318 (+) 2694 WP_098717309.1 bifunctional chitinase/lysozyme -
  R6I08_RS01915 (R6I08_01915) bfd 412487..412681 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  R6I08_RS01920 (R6I08_01920) bfr 412753..413229 (+) 477 WP_000675504.1 bacterioferritin -
  R6I08_RS01925 (R6I08_01925) pilD 413258..413935 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  R6I08_RS01930 (R6I08_01930) gspM 413935..414396 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  R6I08_RS01935 (R6I08_01935) gspL 414393..415556 (-) 1164 WP_001115238.1 type II secretion system protein GspL -
  R6I08_RS01940 (R6I08_01940) gspK 415571..416554 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  R6I08_RS01945 (R6I08_01945) gspJ 416547..417134 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  R6I08_RS01950 (R6I08_01950) gspI 417127..417504 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  R6I08_RS01955 (R6I08_01955) gspH 417501..418010 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  R6I08_RS01960 (R6I08_01960) gspG 418018..418455 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=823142 R6I08_RS01925 WP_000178161.1 413258..413935(-) (pilD) [Escherichia coli isolate FELIX_MS533]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=823142 R6I08_RS01925 WP_000178161.1 413258..413935(-) (pilD) [Escherichia coli isolate FELIX_MS533]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378