Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   QC632_RS01725 Genome accession   NZ_CP123897
Coordinates   396369..397430 (+) Length   353 a.a.
NCBI ID   WP_064024049.1    Uniprot ID   A0A1S1Y3S6
Organism   Methylomonas sp. UP202     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 391369..402430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QC632_RS01705 (QC632_01705) hisG 391442..392074 (+) 633 WP_071158592.1 ATP phosphoribosyltransferase -
  QC632_RS01710 (QC632_01710) hisD 392071..393372 (+) 1302 WP_281022059.1 histidinol dehydrogenase -
  QC632_RS01715 (QC632_01715) rpmE 393529..393729 (+) 201 WP_082885273.1 50S ribosomal protein L31 -
  QC632_RS01720 (QC632_01720) - 393822..396134 (-) 2313 WP_281022060.1 penicillin-binding protein 1A -
  QC632_RS01725 (QC632_01725) pilM 396369..397430 (+) 1062 WP_064024049.1 pilus assembly protein PilM Machinery gene
  QC632_RS01730 (QC632_01730) - 397430..398002 (+) 573 WP_071158586.1 PilN domain-containing protein -
  QC632_RS01735 (QC632_01735) - 397999..398610 (+) 612 WP_064024033.1 type 4a pilus biogenesis protein PilO -
  QC632_RS01740 (QC632_01740) - 398613..399173 (+) 561 WP_082885274.1 pilus assembly protein PilP -
  QC632_RS01745 (QC632_01745) pilQ 399176..401425 (+) 2250 WP_281022061.1 type IV pilus secretin family protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 37952.47 Da        Isoelectric Point: 4.8032

>NTDB_id=822994 QC632_RS01725 WP_064024049.1 396369..397430(+) (pilM) [Methylomonas sp. UP202]
MSWFSSGQSAVLGVDISTAAVKLLELSRVGARYKVESYAVAPLPQDAIVDKNITNVEQIGSVIKTAVKQSGTRAKHACVA
VAGSSVMTKIISMPASLSEAEMEEQIMVEADQYIPYSLDEVNLDFEVQGATKANPQMVDVLLAASRRENIEDRVAALAYA
GLKAVVVDVEAFAMENAFKLLADQLPEAVEHKTVAIADIGATMTSLNILHDGKTVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYVSDVLEPFKKAMLQQIARSLQFFVSSSANRDVDALILAGGCASIAGLDKLVERDLGIASYIANPF
INMALSNKVKPQSLNNDTPAMMIACGLALRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=822994 QC632_RS01725 WP_064024049.1 396369..397430(+) (pilM) [Methylomonas sp. UP202]
ATGAGCTGGTTTAGCAGTGGTCAATCGGCTGTGCTCGGTGTGGATATTAGCACCGCGGCGGTAAAATTGTTGGAGTTAAG
CCGGGTGGGCGCGCGTTACAAAGTCGAGAGCTATGCGGTGGCGCCTTTGCCGCAAGACGCTATCGTCGATAAGAACATTA
CTAACGTAGAGCAAATCGGCAGTGTGATCAAGACCGCCGTCAAGCAATCCGGAACGCGCGCCAAGCACGCTTGCGTGGCG
GTGGCCGGTTCGTCGGTGATGACTAAAATCATTTCCATGCCGGCGTCGTTGTCGGAGGCGGAAATGGAAGAGCAAATCAT
GGTTGAGGCCGATCAGTACATTCCGTATTCCTTGGACGAGGTTAATCTCGATTTCGAAGTGCAGGGCGCTACCAAGGCCA
ACCCGCAAATGGTCGATGTGTTGTTAGCGGCGTCCAGACGTGAAAATATCGAAGACCGAGTGGCGGCCTTGGCTTACGCC
GGACTAAAGGCGGTCGTTGTCGATGTCGAGGCTTTTGCGATGGAAAACGCTTTCAAATTGTTGGCCGATCAATTGCCGGA
GGCTGTCGAACACAAGACGGTTGCGATTGCCGATATCGGCGCCACGATGACCTCGCTGAATATTCTGCATGACGGTAAGA
CCGTCTATACCCGGGAGCAAGGCTTTGGCGGCAAACAGTTAACCGAGGAAATACAGCGTCGCTACGGCTTGTCTTACGAA
GAGGCGGGGTTGGCGAAAAAGCACGGCGGCTTGCCGGACAACTACGTCAGCGATGTGCTGGAACCTTTCAAAAAAGCCAT
GCTGCAACAAATTGCCAGATCGTTACAGTTTTTTGTGTCTTCCAGCGCGAACCGGGATGTCGATGCGTTGATCTTGGCCG
GCGGTTGCGCCTCGATAGCCGGTTTGGACAAATTGGTGGAACGTGATTTGGGCATCGCTTCCTATATCGCCAACCCCTTC
ATCAACATGGCTCTGTCCAACAAGGTTAAGCCGCAGAGCCTGAATAACGATACTCCGGCGATGATGATCGCTTGCGGTTT
GGCGCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S1Y3S6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.708

100

0.507
  comM Acinetobacter nosocomialis M2

50.142

100

0.501
  comM Acinetobacter baylyi ADP1

48.725

100

0.487
  pilM Legionella pneumophila strain ERS1305867

41.926

100

0.419