Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   NRK31_RS02380 Genome accession   NZ_CP123852
Coordinates   446820..447890 (+) Length   356 a.a.
NCBI ID   WP_000776421.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 5812R     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 441820..452890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NRK31_RS02350 - 442243..443706 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  NRK31_RS02355 - 443687..443890 (+) 204 WP_000087561.1 YqgQ family protein -
  NRK31_RS02360 - 443887..444873 (+) 987 WP_000161314.1 ROK family glucokinase -
  NRK31_RS02365 - 444873..445202 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  NRK31_RS02370 - 445199..445822 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  NRK31_RS02375 comGA 445874..446848 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  NRK31_RS02380 comGB 446820..447890 (+) 1071 WP_000776421.1 competence type IV pilus assembly protein ComGB Machinery gene
  NRK31_RS02385 comGC 447904..448215 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  NRK31_RS02390 comGD 448193..448639 (+) 447 WP_072433556.1 competence type IV pilus minor pilin ComGD Machinery gene
  NRK31_RS02395 comGE 448626..448925 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  NRK31_RS02400 comGF 448843..449340 (+) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  NRK31_RS02405 - 449437..449583 (+) 147 WP_001789879.1 hypothetical protein -
  NRK31_RS02410 - 449573..450097 (+) 525 WP_001015121.1 shikimate kinase -
  NRK31_RS02415 gcvT 450256..451347 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  NRK31_RS02420 gcvPA 451367..452713 (+) 1347 WP_042727693.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41429.81 Da        Isoelectric Point: 10.0606

>NTDB_id=822749 NRK31_RS02380 WP_000776421.1 446820..447890(+) (comGB) [Staphylococcus aureus strain 5812R]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQLMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=822749 NRK31_RS02380 WP_000776421.1 446820..447890(+) (comGB) [Staphylococcus aureus strain 5812R]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAATTGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997