Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   R8586_RS00145 Genome accession   NZ_AP026922
Coordinates   25755..27116 (+) Length   453 a.a.
NCBI ID   WP_078157384.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700038     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 20755..32116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8586_RS00110 - 21110..21907 (-) 798 Protein_15 transposase -
  R8586_RS00115 (PC0038_00180) comW 22173..22409 (+) 237 WP_000939548.1 sigma(X)-activator ComW Regulator
  R8586_RS00120 (PC0038_00190) - 22640..23926 (+) 1287 WP_001818679.1 adenylosuccinate synthase -
  R8586_RS00125 (PC0038_00200) tadA 24127..24594 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  R8586_RS00135 (PC0038_00210) - 24781..25224 (+) 444 WP_000701992.1 dUTP diphosphatase -
  R8586_RS00140 (PC0038_00220) - 25226..25741 (+) 516 WP_000691235.1 histidine phosphatase family protein -
  R8586_RS00145 (PC0038_00230) radA 25755..27116 (+) 1362 WP_078157384.1 DNA repair protein RadA Machinery gene
  R8586_RS00150 (PC0038_00240) - 27189..27686 (+) 498 WP_001809263.1 carbonic anhydrase -
  R8586_RS00155 (PC0038_00250) - 27711..28526 (+) 816 WP_000749769.1 PrsW family intramembrane metalloprotease -
  R8586_RS00160 (PC0038_00260) - 28671..29639 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  R8586_RS00165 - 29776..30054 (-) 279 Protein_25 transposase family protein -
  R8586_RS00170 - 30182..31092 (-) 911 Protein_26 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49448.95 Da        Isoelectric Point: 6.5137

>NTDB_id=82267 R8586_RS00145 WP_078157384.1 25755..27116(+) (radA) [Streptococcus pneumoniae strain PZ900700038]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=82267 R8586_RS00145 WP_078157384.1 25755..27116(+) (radA) [Streptococcus pneumoniae strain PZ900700038]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTGCGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGTCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTTTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

AAA domain (AAA_25)

(63-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

99.779

100

0.998
  radA Streptococcus pneumoniae D39

99.779

100

0.998
  radA Streptococcus pneumoniae R6

99.779

100

0.998
  radA Streptococcus pneumoniae TIGR4

99.779

100

0.998
  radA Streptococcus mitis NCTC 12261

99.338

100

0.993
  radA Streptococcus mitis SK321

99.338

100

0.993
  radA/sms Bacillus subtilis subsp. subtilis str. 168

62.914

100

0.629

Multiple sequence alignment