Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R5031_RS16260 Genome accession   NZ_CP137512
Coordinates   3486930..3488273 (-) Length   447 a.a.
NCBI ID   WP_028681150.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain HPA0271     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3481930..3493273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R5031_RS16225 (R5031_16225) thiS 3482164..3482364 (-) 201 WP_003084514.1 sulfur carrier protein ThiS -
  R5031_RS16230 (R5031_16230) - 3482467..3482844 (-) 378 WP_003084513.1 DUF423 domain-containing protein -
  R5031_RS16235 (R5031_16235) mtgA 3482881..3483612 (+) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  R5031_RS16240 (R5031_16240) - 3483617..3484222 (-) 606 WP_003084511.1 hypothetical protein -
  R5031_RS16245 (R5031_16245) rpoH 3484287..3485141 (-) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  R5031_RS16250 (R5031_16250) ftsX 3485255..3486262 (-) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  R5031_RS16255 (R5031_16255) ftsE 3486262..3486933 (-) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  R5031_RS16260 (R5031_16260) pilA 3486930..3488273 (-) 1344 WP_028681150.1 signal recognition particle-docking protein FtsY Machinery gene
  R5031_RS16265 (R5031_16265) - 3488394..3489791 (+) 1398 WP_003084498.1 M16 family metallopeptidase -
  R5031_RS16270 (R5031_16270) - 3489784..3491271 (+) 1488 WP_003102862.1 M16 family metallopeptidase -
  R5031_RS16275 (R5031_16275) rsmD 3491271..3491867 (+) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  R5031_RS16280 (R5031_16280) - 3491870..3492436 (+) 567 WP_003117889.1 hypothetical protein -

Sequence

Protein


Download         Length: 447 a.a.        Molecular weight: 47574.95 Da        Isoelectric Point: 4.6083

>NTDB_id=821767 R5031_RS16260 WP_028681150.1 3486930..3488273(-) (pilA) [Pseudomonas aeruginosa strain HPA0271]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAPVAEA
PLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSASIGEGM
ASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQVDVARE
PYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDAVQAAK
ARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTKLDGTA
KGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1344 bp        

>NTDB_id=821767 R5031_RS16260 WP_028681150.1 3486930..3488273(-) (pilA) [Pseudomonas aeruginosa strain HPA0271]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCGCCGGTTGCCGAAGCA
CCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACCCGAGCCTAT
CCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCTCTGGCCGCCGCTCCGGCAGTCAGCG
AGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGCGCCAGCATCGGCGAAGGCATG
GCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCTGCTGACCGCCGACGT
CGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGGCCGACAGCGGCGCGC
TGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTCGACGTCGCGCGCGAG
CCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGCGAAGAAGCTGCAACT
CGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGCAGGTCTGGGGCGAGC
GCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCGGTGCAGGCCGCCAAG
GCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGATGGAGGAGCTGAAGAA
GGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACGCCGGCACCGGGCAGA
ACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAACTGGACGGCACCGCC
AAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGGCGAGGGGATCGACGA
TCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(157-222)

SRP54-type protein, GTPase domain (SRP54)

(242-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.454

96.644

0.459