Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QEP15_RS00645 Genome accession   NZ_CP123364
Coordinates   142116..143561 (+) Length   481 a.a.
NCBI ID   WP_056327751.1    Uniprot ID   -
Organism   Achromobacter mucicolens strain Y3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 137116..148561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEP15_RS00630 (QEP15_00630) - 137702..139030 (+) 1329 WP_279989920.1 amidase -
  QEP15_RS00635 (QEP15_00635) leuA 139227..140936 (-) 1710 WP_062840150.1 2-isopropylmalate synthase -
  QEP15_RS00640 (QEP15_00640) - 141455..142117 (-) 663 WP_217618946.1 hypothetical protein -
  QEP15_RS00645 (QEP15_00645) rpoN/rpoN1 142116..143561 (+) 1446 WP_056327751.1 RNA polymerase factor sigma-54 Machinery gene
  QEP15_RS00650 (QEP15_00650) - 143683..143961 (+) 279 WP_056327752.1 bacterioferritin-associated ferredoxin -
  QEP15_RS00655 (QEP15_00655) - 143991..144854 (-) 864 WP_280148311.1 LysR substrate-binding domain-containing protein -
  QEP15_RS00660 (QEP15_00660) - 144987..145898 (+) 912 WP_175156346.1 DMT family transporter -
  QEP15_RS00665 (QEP15_00665) bfr 146274..146750 (+) 477 WP_056327755.1 bacterioferritin -
  QEP15_RS00670 (QEP15_00670) - 146844..147518 (-) 675 WP_056327756.1 hypothetical protein -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52645.98 Da        Isoelectric Point: 4.7656

>NTDB_id=820710 QEP15_RS00645 WP_056327751.1 142116..143561(+) (rpoN/rpoN1) [Achromobacter mucicolens strain Y3]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLDLEAEISQVLAENPLLDREDEPATAEDRAESERESSVQDDEP
AAERETAIDEMPGSGGVYPDDDASLPEAARPDTLREHLLGQLALTRAAPRDAALASLLIDELDENGYLGSPLDEILGWLP
AEMEPDMDELRAALSLLQSFDPVGIGARDMAECLQLQLRNPDLTLLPEAADPVVLACARRICAEHLPLLATGNAARLCAA
VGCDETTFRAAHSLILRLEPRPGRAWTVPAAEYAVPDVIVRKTRRGWQATLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QAQLQQAKWMIRNVEQRFDTILRVSQAIVERQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGTGVSTEGGDATSATAVQTYIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQASA
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=820710 QEP15_RS00645 WP_056327751.1 142116..143561(+) (rpoN/rpoN1) [Achromobacter mucicolens strain Y3]
ATGCAAAATATTGTACCTATGACCCGTCCAATTTTAGAACTGCGGCCTGGCCAGCACCTGACGCTGACCCCACAACTTCA
ACAATCGATCCGATTGTTGCAGTTGTCCACGCTCGACCTTGAAGCGGAAATCTCGCAAGTGCTGGCCGAGAATCCCCTGC
TGGATCGCGAGGACGAGCCGGCGACGGCGGAGGATCGCGCCGAATCCGAGCGCGAATCTTCGGTTCAGGACGACGAACCG
GCGGCCGAGCGCGAGACCGCCATCGACGAGATGCCTGGCTCGGGCGGGGTGTATCCCGACGACGACGCCAGCCTGCCCGA
GGCGGCGCGGCCGGACACGCTGCGCGAACATCTGCTGGGTCAGCTTGCCCTGACGCGCGCCGCGCCGCGCGATGCCGCGC
TGGCGTCCCTGTTGATCGACGAACTGGACGAAAACGGCTACCTGGGTTCGCCGCTGGACGAAATCCTGGGTTGGCTCCCT
GCCGAAATGGAGCCCGACATGGACGAGCTGCGCGCCGCCCTGTCGCTTCTGCAATCGTTCGACCCGGTGGGCATCGGCGC
GCGCGACATGGCTGAATGCCTGCAACTGCAACTGCGCAATCCCGATCTCACCTTGCTGCCGGAGGCGGCTGACCCCGTCG
TGCTGGCCTGTGCGCGCCGGATCTGCGCCGAGCACCTGCCGCTGTTGGCAACCGGCAACGCCGCGCGCCTGTGTGCGGCC
GTCGGCTGCGACGAGACCACGTTCCGCGCGGCGCATTCGCTGATCTTGCGCCTGGAACCCCGGCCGGGTCGGGCCTGGAC
CGTGCCGGCGGCCGAGTACGCAGTGCCGGACGTCATCGTCCGCAAGACGCGGCGCGGCTGGCAGGCCACGCTGAATAGCG
CCGCGGTGCCGCGGCTGCAGATCAATGGGCTGTACGCCCAGATGCTGGGCAACCAAAAGGAATCCGCCCACGCCGGCCTG
CAGGCCCAGCTGCAGCAGGCCAAATGGATGATCCGCAACGTGGAGCAGCGCTTTGACACGATCCTGCGCGTGTCGCAGGC
CATCGTCGAGCGTCAGACGGCTTTCTTCAGCCAGGGGCCGGCGGCGATGCGTCCGCTCATCCTGAAGGACATCGCCGGCG
AGCTGGGTTTGCATGAATCGACCATTTCCCGGGCCACAACACAGAAGTACATGCTCACTCCATTCGGCACGCTGGAGCTC
AAGCGCTTCTTTGGAACGGGCGTGTCGACAGAAGGCGGCGACGCCACGTCGGCCACTGCCGTGCAGACCTACATCCGCCA
AATGGTGGCCGACGAGAATCGCGCCAAGCCGTTGTCGGATAGCCAGATCATGCAAAAACTGGCCGATCAGGGGATAGTGA
TTGCGCGCCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGGATTGCCCCCGCCGCGCTGCGCAAGGCGCAGGCCTCCGCG
CGCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

48.907

100

0.511