Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   R4V96_RS19105 Genome accession   NZ_CP137162
Coordinates   3921813..3922253 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain YA-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3916813..3927253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4V96_RS19085 (R4V96_19070) aroP 3917811..3919184 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  R4V96_RS19090 (R4V96_19075) ampE 3919227..3920081 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  R4V96_RS19095 (R4V96_19080) ampD 3920078..3920629 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R4V96_RS19100 (R4V96_19085) nadC 3920717..3921610 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R4V96_RS19105 (R4V96_19090) pilA 3921813..3922253 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  R4V96_RS19110 (R4V96_19095) pilB 3922263..3923648 (+) 1386 WP_001025173.1 type II secretion system protein GspE Machinery gene
  R4V96_RS19115 (R4V96_19100) hofC 3923638..3924840 (+) 1203 WP_000157234.1 protein transport protein HofC -
  R4V96_RS19120 (R4V96_19105) guaC 3924875..3925918 (-) 1044 WP_001217338.1 GMP reductase -
  R4V96_RS19125 (R4V96_19110) - 3926074..3926118 (-) 45 WP_120795372.1 protein YacM -
  R4V96_RS19130 (R4V96_19115) coaE 3926143..3926763 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=820505 R4V96_RS19105 WP_000360895.1 3921813..3922253(+) (pilA) [Escherichia coli strain YA-1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=820505 R4V96_RS19105 WP_000360895.1 3921813..3922253(+) (pilA) [Escherichia coli strain YA-1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363