Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   R4540_RS10455 Genome accession   NZ_CP137135
Coordinates   2320672..2322135 (+) Length   487 a.a.
NCBI ID   WP_000804830.1    Uniprot ID   Q9KP49
Organism   Vibrio cholerae V52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2315672..2327135
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4540_RS10425 (R4540_10420) - 2316304..2317266 (+) 963 WP_000904329.1 calcium/sodium antiporter -
  R4540_RS10430 (R4540_10425) kdsD 2317322..2318296 (+) 975 WP_001120616.1 arabinose-5-phosphate isomerase KdsD -
  R4540_RS10435 (R4540_10430) kdsC 2318296..2318853 (+) 558 WP_000099768.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  R4540_RS10440 (R4540_10435) lptC 2318850..2319413 (+) 564 WP_000059348.1 LPS export ABC transporter periplasmic protein LptC -
  R4540_RS10445 (R4540_10440) lptA 2319394..2319888 (+) 495 WP_000830444.1 lipopolysaccharide transport periplasmic protein LptA -
  R4540_RS10450 (R4540_10445) lptB 2319891..2320616 (+) 726 WP_000996178.1 LPS export ABC transporter ATP-binding protein -
  R4540_RS10455 (R4540_10450) rpoN/rpoN1 2320672..2322135 (+) 1464 WP_000804830.1 RNA polymerase factor sigma-54 Machinery gene
  R4540_RS10460 (R4540_10455) hpf 2322160..2322447 (+) 288 WP_001166643.1 ribosome hibernation promoting factor -
  R4540_RS10465 (R4540_10460) ptsN 2322450..2322896 (+) 447 WP_001177164.1 PTS IIA-like nitrogen regulatory protein PtsN -
  R4540_RS10470 (R4540_10465) rapZ 2322909..2323769 (+) 861 WP_001237198.1 RNase adapter RapZ -
  R4540_RS10475 (R4540_10470) - 2323778..2324056 (+) 279 WP_001135899.1 HPr family phosphocarrier protein -
  R4540_RS10480 (R4540_10475) mgtE 2324217..2325578 (+) 1362 WP_000840452.1 magnesium transporter -
  R4540_RS10485 (R4540_10480) pmbA 2325651..2326994 (-) 1344 WP_000382639.1 metalloprotease PmbA -

Sequence

Protein


Download         Length: 487 a.a.        Molecular weight: 55438.47 Da        Isoelectric Point: 4.2824

>NTDB_id=820348 R4540_RS10455 WP_000804830.1 2320672..2322135(+) (rpoN/rpoN1) [Vibrio cholerae V52]
MKPSLQLKLGQQLAMTPQLQQAIRLLQLSTLDLQQEIQEALESNPLLEVEENQDEAPSLDSVRMTEESPREPEELYEPEP
QDSSDLIEKSEISAELEMDTTWDEVYSANTGSTGLALDDDAPIYQGETTQTLQDYLHWQLDLTPFSDVDRTIAVALIDAI
DDYGYLTVSLEEIQESLRSDDIELDEIEAVRKRIQQFDPFGVASLNLQDCLLLQLTTYPCDTPWLEEARLLLSQYIDDLG
NRDYKTILKETKLKEEDLREILQLIQQLDPRPGSRIAQDHAEYVIPDVSVYKEQGRWLVTINPDSVPKLKINQQYADLMR
GNNAESNYIRTNLQEAKWLIKSLESRNETLLKVAKCIVEHQHDFFEYGEEAMKPMVLNDVAMAVEMHESTISRVTTQKYM
HTPRGIFELKYFFSSHVSTDNGGECSSTAIRALIKKLVAAENPAKPLSDSKIATLLADQGIQVARRTIAKYRESLGIAPS
SQRKRLL

Nucleotide


Download         Length: 1464 bp        

>NTDB_id=820348 R4540_RS10455 WP_000804830.1 2320672..2322135(+) (rpoN/rpoN1) [Vibrio cholerae V52]
ATGAAACCGTCATTACAGCTCAAGCTAGGTCAACAGTTAGCCATGACTCCGCAGCTCCAGCAGGCGATTCGTCTGTTGCA
GCTTTCGACGCTGGATCTGCAGCAAGAAATTCAGGAAGCATTGGAATCTAACCCCTTGTTAGAGGTGGAAGAGAACCAAG
ACGAAGCGCCATCGCTTGATAGCGTACGTATGACGGAAGAAAGCCCGCGCGAGCCAGAAGAACTCTACGAACCTGAACCG
CAGGATAGCTCGGATCTGATTGAAAAATCGGAAATCAGCGCTGAATTAGAGATGGATACCACTTGGGATGAAGTCTACAG
CGCCAATACTGGCAGTACAGGACTGGCACTCGATGATGATGCCCCTATCTACCAAGGCGAAACCACCCAAACCCTGCAAG
ATTATCTTCACTGGCAACTCGACTTAACTCCCTTTAGCGATGTGGATCGCACCATTGCCGTTGCACTTATCGATGCTATC
GACGATTACGGTTATTTAACCGTCTCACTAGAGGAGATCCAAGAGAGCCTGCGCAGTGATGACATTGAGTTGGATGAGAT
TGAAGCGGTACGTAAACGCATTCAGCAGTTTGACCCCTTTGGTGTGGCATCGCTTAATCTGCAAGACTGCTTGTTGCTGC
AACTCACCACCTATCCCTGTGATACTCCTTGGCTGGAAGAAGCGCGTTTACTGCTCTCACAGTACATCGATGATTTAGGG
AATCGCGATTACAAAACCATTCTGAAAGAGACTAAGCTCAAAGAAGAGGACTTGCGCGAGATCCTGCAACTCATCCAACA
GCTCGATCCTCGCCCCGGCAGTCGAATTGCCCAAGATCACGCTGAATACGTTATACCTGACGTGTCAGTCTATAAAGAAC
AAGGTCGATGGCTGGTCACCATCAACCCAGATAGTGTGCCTAAACTGAAGATCAATCAGCAGTATGCCGACCTGATGCGC
GGTAATAATGCGGAGAGCAACTACATCCGTACCAATTTGCAAGAGGCAAAATGGCTGATCAAAAGTTTAGAAAGTAGAAA
CGAGACACTGCTCAAAGTTGCCAAGTGCATAGTTGAACACCAACACGATTTCTTCGAGTATGGAGAAGAGGCGATGAAGC
CGATGGTGCTCAACGATGTGGCGATGGCGGTGGAAATGCATGAATCGACAATTTCGCGTGTCACCACGCAGAAATACATG
CATACCCCGCGCGGCATTTTTGAGTTGAAGTACTTTTTCTCCAGCCACGTCAGTACTGACAATGGCGGAGAGTGCTCGTC
CACTGCGATCAGGGCGCTGATCAAAAAACTGGTGGCGGCAGAGAATCCTGCCAAACCACTCAGTGACAGCAAGATCGCAA
CCCTACTCGCTGACCAAGGCATCCAAGTCGCGCGGCGAACCATCGCCAAATATCGTGAATCACTTGGCATCGCCCCATCA
AGTCAGCGCAAACGCCTGCTATAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(327-485)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(128-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KP49

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.533

100

0.46