Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   QAO42_RS10025 Genome accession   NZ_CP123139
Coordinates   2015538..2016887 (+) Length   449 a.a.
NCBI ID   WP_000815638.1    Uniprot ID   Q53IL8
Organism   Staphylococcus aureus strain GE-MRSA22     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2008687..2025302 2015538..2016887 within 0


Gene organization within MGE regions


Location: 2008687..2025302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO42_RS09995 (QAO42_09960) - 2008687..2009184 (-) 498 WP_000051479.1 hypothetical protein -
  QAO42_RS10000 (QAO42_09965) - 2009653..2010030 (-) 378 WP_000687965.1 hypothetical protein -
  QAO42_RS10005 (QAO42_09970) - 2010456..2011388 (-) 933 WP_000364405.1 hypothetical protein -
  QAO42_RS10010 (QAO42_09975) - 2011460..2013043 (-) 1584 WP_000271876.1 hypothetical protein -
  QAO42_RS10015 (QAO42_09980) ssb 2013215..2013511 (+) 297 WP_000221966.1 cassette chromosome ssDNA-binding protein -
  QAO42_RS10020 (QAO42_09985) cch1 2013511..2015304 (+) 1794 WP_000195099.1 cassette chromosome replicative helicase -
  QAO42_RS10025 (QAO42_09990) ccrA 2015538..2016887 (+) 1350 WP_000815638.1 cassette chromosome recombinase CcrA Machinery gene
  QAO42_RS10030 (QAO42_09995) ccrB 2016909..2018537 (+) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  QAO42_RS10035 (QAO42_10000) - 2019059..2019409 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  QAO42_RS10040 (QAO42_10005) - 2019496..2019807 (+) 312 WP_000700851.1 DUF960 family protein -
  QAO42_RS10045 (QAO42_10010) - 2019822..2020328 (+) 507 WP_001092188.1 DUF1643 domain-containing protein -
  QAO42_RS10050 (QAO42_10015) - 2020377..2021987 (+) 1611 Protein_1947 IS1182 family transposase -
  QAO42_RS10055 (QAO42_10020) - 2021978..2022220 (-) 243 Protein_1948 type I restriction enzyme endonuclease domain-containing protein -
  QAO42_RS10060 (QAO42_10025) - 2022210..2023196 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  QAO42_RS10065 (QAO42_10030) mecA 2023293..2025302 (+) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52636.23 Da        Isoelectric Point: 9.9639

>NTDB_id=819413 QAO42_RS10025 WP_000815638.1 2015538..2016887(+) (ccrA) [Staphylococcus aureus strain GE-MRSA22]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
HGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYCGRVNNQYGQFDNMFPSIVSTSI
YEQAQRLRSQKQTKQTSSDNQLKQKIKCPCCNATLTNMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHIIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=819413 QAO42_RS10025 WP_000815638.1 2015538..2016887(+) (ccrA) [Staphylococcus aureus strain GE-MRSA22]
ATGAAACAAGTCATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAAGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGTCGCAAAGATAATCGTAGTGGGT
ATCGACAAATAACACAATTAATTCAACAAGGACAGTGTGACATATTATGCTGTTATCGTCTTAATAGGTTGCATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACGTACCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCGGATAACATTGGAGAAC
AAGTCAGAAATGGGCTTCAAGAAAAAGCAAAGCAAGGTCGATTGATTACAACACATGCACCTTTTGGTTACGATTATCAC
CACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGCCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCCTATCAAGTTCGTAACATTATCATCA
ATCCTAATTATTGTGGTCGTGTCAACAATCAATATGGCCAATTCGACAATATGTTTCCTTCTATTGTTTCCACAAGTATA
TATGAGCAAGCGCAGAGACTTCGATCGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCATTAGAAAAAAGAATCATACATTACGTTACTACGTCTGTCCTA
AAAACATGAATGCTTCACGCTTTGTCTGTGATTTTAAAGGCATCAATGCACAAACACTTGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAAGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATTGATGCAGAAACGTTCA
GAGAGCAAACACAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGGCATCAAATACAACACATCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATTGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(169-250)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q53IL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

97.55

100

0.976
  ccrA Staphylococcus aureus COL

75.501

100

0.755