Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   QAO28_RS02720 Genome accession   NZ_CP123104
Coordinates   495292..496266 (+) Length   324 a.a.
NCBI ID   WP_069995863.1    Uniprot ID   -
Organism   Staphylococcus aureus strain IT-MSSA53     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490292..501266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAO28_RS02685 (QAO28_02685) rpmG 490752..490901 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  QAO28_RS02690 (QAO28_02690) - 491110..491640 (+) 531 WP_072384884.1 5-formyltetrahydrofolate cyclo-ligase -
  QAO28_RS02695 (QAO28_02695) - 491661..493124 (+) 1464 WP_001017867.1 rhomboid family protein -
  QAO28_RS02700 (QAO28_02700) - 493105..493308 (+) 204 WP_000087561.1 YqgQ family protein -
  QAO28_RS02705 (QAO28_02705) - 493305..494291 (+) 987 WP_000161314.1 ROK family glucokinase -
  QAO28_RS02710 (QAO28_02710) - 494291..494620 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  QAO28_RS02715 (QAO28_02715) - 494617..495240 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  QAO28_RS02720 (QAO28_02720) comGA 495292..496266 (+) 975 WP_069995863.1 competence type IV pilus ATPase ComGA Machinery gene
  QAO28_RS02725 (QAO28_02725) comGB 496238..497308 (+) 1071 WP_045176802.1 competence type IV pilus assembly protein ComGB Machinery gene
  QAO28_RS02730 (QAO28_02730) comGC 497322..497633 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  QAO28_RS02735 (QAO28_02735) comGD 497611..498057 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  QAO28_RS02740 (QAO28_02740) comGE 498044..498343 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  QAO28_RS02745 (QAO28_02745) comGF 498261..498758 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  QAO28_RS02750 (QAO28_02750) - 498855..499001 (+) 147 WP_001789879.1 hypothetical protein -
  QAO28_RS02755 (QAO28_02755) - 498991..499515 (+) 525 WP_001015121.1 shikimate kinase -
  QAO28_RS02760 (QAO28_02760) gcvT 499674..500765 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36824.93 Da        Isoelectric Point: 8.8508

>NTDB_id=818764 QAO28_RS02720 WP_069995863.1 495292..496266(+) (comGA) [Staphylococcus aureus strain IT-MSSA53]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLINKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=818764 QAO28_RS02720 WP_069995863.1 495292..496266(+) (comGA) [Staphylococcus aureus strain IT-MSSA53]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATTAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.074

100

0.991
  comGA Staphylococcus aureus N315

99.074

100

0.991