Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   QAY74_RS09965 Genome accession   NZ_CP123093
Coordinates   2009999..2011627 (+) Length   542 a.a.
NCBI ID   WP_001186608.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MRSA-EDCC5443     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2001777..2018392 2009999..2011627 within 0


Gene organization within MGE regions


Location: 2001777..2018392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QAY74_RS09930 (QAY74_09905) - 2001777..2002274 (-) 498 WP_000051479.1 hypothetical protein -
  QAY74_RS09935 (QAY74_09910) - 2002743..2003120 (-) 378 WP_000687965.1 hypothetical protein -
  QAY74_RS09940 (QAY74_09915) - 2003546..2004478 (-) 933 WP_000364405.1 hypothetical protein -
  QAY74_RS09945 (QAY74_09920) - 2004550..2006133 (-) 1584 WP_000271876.1 hypothetical protein -
  QAY74_RS09950 (QAY74_09925) ssb 2006305..2006601 (+) 297 WP_000221966.1 cassette chromosome ssDNA-binding protein -
  QAY74_RS09955 (QAY74_09930) cch1 2006601..2008394 (+) 1794 WP_000195099.1 cassette chromosome replicative helicase -
  QAY74_RS09960 (QAY74_09935) ccrA 2008628..2009977 (+) 1350 WP_000815638.1 cassette chromosome recombinase CcrA Machinery gene
  QAY74_RS09965 (QAY74_09940) ccrB 2009999..2011627 (+) 1629 WP_001186608.1 cassette chromosome recombinase CcrB Machinery gene
  QAY74_RS09970 (QAY74_09945) - 2012149..2012499 (+) 351 WP_000849672.1 SAUGI family uracil-DNA glycosylase inhibitor -
  QAY74_RS09975 (QAY74_09950) - 2012586..2012897 (+) 312 WP_000700851.1 DUF960 family protein -
  QAY74_RS09980 (QAY74_09955) - 2012912..2013418 (+) 507 WP_001092188.1 DUF1643 domain-containing protein -
  QAY74_RS09985 (QAY74_09960) - 2013467..2015077 (+) 1611 Protein_1933 IS1182 family transposase -
  QAY74_RS09990 (QAY74_09965) - 2015068..2015310 (-) 243 Protein_1934 type I restriction enzyme endonuclease domain-containing protein -
  QAY74_RS09995 (QAY74_09970) - 2015300..2016286 (-) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  QAY74_RS10000 (QAY74_09975) mecA 2016383..2018392 (+) 2010 WP_411905185.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62654.83 Da        Isoelectric Point: 9.7970

>NTDB_id=818572 QAY74_RS09965 WP_001186608.1 2009999..2011627(+) (ccrB) [Staphylococcus aureus strain MRSA-EDCC5443]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILANPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDIAYKQQQYD
EVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNEDKTLFDAKEISKLLQHIFHDIKHIEKSRLK
ALYLSVIDRIDIKKDGNHKKQFYVTLKLNNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=818572 QAY74_RS09965 WP_001186608.1 2009999..2011627(+) (ccrB) [Staphylococcus aureus strain MRSA-EDCC5443]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAAGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAACAGACCTGAGCTCCAACGTATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
GTATACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTCAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAACGTATTTATGGGTCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAACACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTGCTATGCCAAAGGACATGGCTATCGTAAAATTGCCAATGCATTGAATCACAAAGGATATGTCACTAAAA
AGGGGAAACCTTTCAGTATTAGTTCAATCACATACATCTTAGCTAACCCTTTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAGAAACAAGTCAGTCAAAAACCCCAAGTCCATGGCAAAGGAACGA
ATCTGCTTACAGGCATTATTCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACCACGAATACTCTTAAAGAC
GGGACTAAGAAACGTATTCGTTACTATTCATGTAGTAATTTTCGGAACAAGGGTTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTTGAAATAGTCAAAAGTGATAAAGTCATTCAACGCGTAG
TAACACACGTCAATCAAGAAAATCAAGTTGATGGCGCTGCACTTCATCACGATATTGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAAATAAATAACTTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGACCAAGTAT
TCAAAAATATGAAAAGCAACTCAATGACATTACGAATCAAATCAACCAACTCAAAAATCAACAAAATGAAGATAAGACTT
TATTTGATGCCAAAGAAATCAGTAAACTATTACAACACATCTTTCATGATATTAAACACATAGAAAAATCTCGACTCAAA
GCATTGTATCTATCAGTGATTGATCGCATTGATATTAAAAAAGATGGTAATCATAAAAAACAATTCTATGTCACACTCAA
ACTTAATAACGAAATCATTAAACAACTTTTCAATAATAAACAACTCGACGAAGTGCATCTCAGCACTTCGTCTTTATTTT
TGCCCCAAACACTATATCTTACTATTTAG

Domains

Predicted by InterproScan.

Recombinase (Recombinase)

(180-280)

Resolvase, N terminal domain (Resolvase)

(11-156)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

98.893

100

0.989